Effect of photobiomodulation therapy on the proliferation phase and wound healing in rats fed with an experimental hypoproteic diet

Author(s)

Amadio, Eliane Martins ^[1] ; Marcos, Rodrigo Labat ^[2] ; Serra, Andrey Jorge ^{[2, 3]} ; dos Santos, Solange Almeida ^[1] ; Caires, Jheniphe Rocha ^[1] ; Fernandes, Guilherme Henrique Cardosos ^[1] ; Leal-Junior, Ernesto Cesar ^[1] ; Ferrari, Joao Carlos Correa ^[1] ; de Tarso Camillo de Carvalho, Paulo ^{[1, 2]}

Affiliation

^[1] Univ Nove Julho UNINOVE, Postgrad Program Rehabil Sci, Sao Paulo, SP - Brazil

^[2] Nine July Univ, Nove Julho UNINOVE, Postgrad Program Appl Biophoton Hlth Sci, Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Postgrad Program Cardiol, Rua Pedro Toledo 781, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 36, n. 7, p. 1427-1435, SEP 2021.
• Web of Science Citations: 0

Abstract

Photobiomodulation therapy (PBMT) has been indicated for enforcement on healing skin wounds. This study evaluated the effects of PBMT on the healing of skin wounds during the proliferation phase in rats with a hypoproteic diet. Rats were randomized to one of the following groups (n = 10 per group): (i) injured normoproteic (25% protein) not subjected to PBMT; (ii) injured normoproteic who received PBMT; (iii) injured hypoproteic (8% protein) not subjected to PBMT; and (iv) injured hypoproteic who received PBMT. Rats were submitted to skin wounds and then treated with PBMT (low-level laser therapy: 660 nm, 50 mW, 1.07 W/cm(2), 0.028 cm(2), 72 J/cm(2), 2 J). Analyses were performed at 7 and 14 days of follow-up: semi-quantitative histopathologic analysis, collagen type I and III expressions, immunohistochemical marking for matrix metalloproteinases-3 (MMP-3) and (matrix metalloproteinases-9) MMP-9, and mechanical resistance test. There were significant differences between the normoproteic groups and their respective treated groups (p < 0.05), as well as to treated and untreated hypoproteic groups in histopathologic analysis semi-quantitatively and immunohistochemistry for MMP-3 and 9, in which PBMT was able to decrease immunostaining. Moreover, there was a decrease in collagen deposition with the statistical difference (p < 0.05) for both collagen types III and I. In conclusion, PBMT application was proved effective in the treatment of cutaneous wounds in rats submitted to a hypoproteic diet. These alterations were more salient in the proliferation stage with the reduction of metalloproteinases providing better mechanical resistance of the injured area in the remodeling phase with an intensification of type I collagen. (AU)

• FAPESP's process: 18/26062-6 - Analysis of the effects of photobiomodulation on the activity of chondrocytes and articular cartilage in experimental model of arthritis induced by collagen: in vivo and in vitro study
• Grantee: Rodrigo Labat Marcos
• Support Opportunities: Regular Research Grants