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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

oes maternal exposure to nicotine affect the oocyte quality and reproductive capacity in adult offspring

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Author(s):
Paccola, C. C. [1] ; Souza, G. S. [1] ; Freitas, I. M. M. [1] ; Souza, J. C. [1] ; Martins, L. L. [1] ; Vendramini, V. [1] ; Miraglia, S. M. [1]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Paulo, Dept Morphol & Genet, Dev Biol Lab, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Toxicology and Applied Pharmacology; v. 426, SEP 1 2021.
Web of Science Citations: 0
Abstract

Gonadal development begins in the intrauterine phase and females from most species are born with an established oocyte reserve. Exposure to drugs during gestation can compromise the offspring health, also affecting the gametes quality. Nicotine, the main component of cigarettes, is an oxidant agent capable of altering the fertility in men and women. As female gametes are susceptible to oxidative stress, this drug can damage the oolemma and affect oocyte maturation, induce errors during chromosomal segregation and DNA fragmentation. Oocyte mitochondria are particularly susceptible to injuries, contributing to the oocyte quality loss and embryonic development disruption. Thus, considering the high number of women who smoke during pregnancy, while significant events are occurring in the embryo for future fertility of offspring, we seek to verify the quality of the oocytes from adult rats exposed to nicotine during intrauterine phase and breastfeeding. Pregnant Wistar rats received nicotine by osmotic mini-pumps and the female progenies were evaluated in adulthood for oocyte quality (viability, lipid peroxidation, generation of reactive oxygen species and mitochondrial integrity) and reproductive capacity. Embryos (3dpc) and fetuses (20dpc) generated by these rats were also evaluated. The results showed that the dose of 2 mg/kg/day of nicotine through placenta and breast milk does not affect the number of oocytes and the fertility capacity of adult rats. However, it causes some morphological alterations in oocytes, mitochondrial changes, embryonic fragmentation and disruption of fetal development. The malformations in fetuses generated from these gametes can also indicate the occurrence of epigenetic modifications. (AU)

FAPESP's process: 19/08976-3 - Analysis of oocyte quality and reproductive capacity of rats exposed to nicotine: a comparison between damage after exposure in adulthood and in the intrauterine and lactation phases
Grantee:Izabela Maria Mota de Freitas
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 17/06668-4 - Effect of Intrauterine and breast-feeding nicotine exposure on oocytes quality and reproductive capacity of adult rats
Grantee:Camila Cicconi Paccola
Support Opportunities: Regular Research Grants