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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Compartmentalization of therapeutic proteins into semi-crystalline PEG-PCL polymersomes

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Author(s):
Pachioni-Vasconcelos, Juliana de Almeida [1] ; Apolinario, Alexsandra Conceicao [1] ; Lopes, Andre Moreni [2] ; Pessoa, Jr., Adalberto [1] ; Barbosa, Leandro Ramos Souza [3] ; Rangel-Yagui, Carlota de Oliveira [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Sao Paulo - Brazil
[2] Univ Estadual Campinas, Fac Pharmaceut Sci, Campinas - Brazil
[3] Univ Sao Paulo, Inst Phys, Dept Gen Phys, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: SOFT MATERIALS; v. 19, n. 2, p. 222-230, APR 3 2021.
Web of Science Citations: 2
Abstract

Polymersomes are self-assembled nanostructures with high loading capacity, possibility to deliver hydrophilic as well as hydrophobic drugs and stealth characteristic resulting in low immunogenicity. These vesicles can be used to deliver enzyme drugs such as L-asparaginase (ASNase), a first line drug for acute lymphoblastic leukemia treatment. Here, polymersomes based on three semi-crystalline copolymers of poly(ethylene glycol)-b-poly(epsilon-caprolactone), namely PEG(45)PCL(44), PEG(114)PCL(98), and PEG(114)PCL(114), were investigated for the encapsulation of ASNase, as well as of bovine serum albumin (BSA) as a model protein. Critical aggregation concentration (CAC) of the copolymers was determined by fluorescence spectroscopy and the values varied from 0.6 to 1.26 mg/L. Using film hydration, polymersomes of 200-400 nm and narrow size distribution (polydispersity index values of 0.2-0.3) were obtained when centrifugation was used as a post-film technique. The encapsulation efficiency (EE %) was determined either after centrifugation of the suspension, followed by the proteins measurement in the supernatant or after Size Exclusion Chromatography (SEC) purification, in which the quantification was performed in the eluted fractions corresponding to the free protein. Higher encapsulation efficiency values were obtained after centrifugation (EE% approximate to 20%) in comparison to the measurements after SEC (EE% approximate to 1-5%), indicating that the protein could be partially entrapped in the polymeric network when centrifugation is used as separation method. Nonetheless, this system could provide an initial effect of the free ASNase followed by a long-term effect based on the encapsulated enzyme, leading to decreased dose administration. (AU)

FAPESP's process: 13/08617-7 - Production of extracellular L-asparaginase: from bioprospecting to the engineering of an antileukemic biopharmaceutical
Grantee:Adalberto Pessoa Junior
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/10456-4 - Development and characterization of poly(ethylene glycol) methyl ether-Block-Poly (D,L-Lactide) (PEG-PLA) Polymersomes for the release of recombinant L-asparaginase
Grantee:Alexsandra Conceição Apolinário
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 17/10789-1 - Combinatorial therapy using polymersomes decorated with transferrin and incorporated into chitosan hydrogels as smart drug delivery systems for melanoma tumor cells
Grantee:André Moreni Lopes
Support Opportunities: Research Grants - Young Investigators Grants