| Full text | |
| Author(s): |
Pavani, Raphael S.
[1, 2]
;
de Lima, Loyze P.
[1, 2]
;
Lima, Andre A.
[1, 2]
;
Fernandes, Carlos A. H.
[3, 4]
;
Fragoso, Stenio P.
[5]
;
Calderano, Simone G.
[6, 1]
;
Elias, Maria Carolina
[1, 2]
Total Authors: 7
|
| Affiliation: | [1] Inst Butantan, Ctr Toxins Immune Response & Cell Signaling CeTIC, Sao Paulo - Brazil
[2] Inst Butantan, Lab Ciclo Celular, Sao Paulo - Brazil
[3] Ecole Normale Super Paris Saclay, Lab Biol & Pharmacol Appl, Cachan - France
[4] Univ Estadual Paulista Julio de Mesquita Filho UN, Inst Biociencias, Dept Fis & Biofis, Botucatu, SP - Brazil
[5] Fiocruz MS, Inst Carlos Chagas, Curitiba, Parana - Brazil
[6] Inst Butantan, Lab Parasitol, Sao Paulo - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | FEBS Letters; v. 594, n. 10, p. 1596-1607, MAY 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Replication protein A (RPA), a heterotrimeric complex, is the major single-stranded DNA binding protein in eukaryotes. Recently, we characterized RPA from Trypanosoma cruzi, showing that it is involved in DNA replication and DNA damage response in this organism. Better efficiency in differentiation from epimastigote to metacyclic trypomastigote forms was observed in TcRPA-2 subunit heterozygous knockout cells, suggesting that RPA is involved in this process. Here, we show that RPA cellular localization changes during the T. cruzi life cycle, with RPA being detected only in the cytoplasm of the metacyclic and bloodstream trypomastigotes. We also identify a nuclear export signal (NES) in the trypanosomatid RPA-2 subunit. Mutations in the negatively charged residues of RPA-2 NES impair the differentiation process, suggesting that RPA exportation affects parasite differentiation into infective forms. (AU) | |
| FAPESP's process: | 16/50050-2 - How do common and diverged features of the replicative stress response shape the biology of TriTryp parasites? |
| Grantee: | Maria Carolina Quartim Barbosa Elias Sabbaga |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/02978-0 - Functional analysis of RPA complex in Trypanosoma cruzi and its involvement with telomeric DNA |
| Grantee: | Raphael Souza Pavani |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 18/14432-3 - A network for an integrative biology in neglected diseases: bridging epigenetics, metabolism and cell cycle in pathogenic trypanosomatids |
| Grantee: | Ariel Mariano Silber |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 18/21785-0 - Analysis of Orc1/Cdc6-DNA interaction during the life cycle of Trypanosoma cruzi |
| Grantee: | Loyze Paola Oliveira de Lima |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling |
| Grantee: | Hugo Aguirre Armelin |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |