ntineoplastic agents aggravate the damages caused by nicotine on the peri-implant bone: an in vivo histomorphometric and immunohistochemical study in rat

Author(s)

de Almeida, Juliano Milanezi ^{[1, 2]} ; Ervolino, Edilson ^{[3, 2]} ; Gusman, David Jonathan Rodrigues ^{[1, 2]} ; Fiorin, Luiz Guilherme ^{[1, 2]} ; Alves, Breno Edson Sendao ^{[1, 2]} ; Guastaldi, Fernando Pozzi Semeghini ^[4] ; Matheus, Henrique Rinaldi ^{[1, 2]}

Affiliation

^[1] Sao Paulo State Univ Unesp, Sch Dent, Dept Diag & Surg, Periodont Div, St Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[2] Sao Paulo State Univ Unesp, Sch Dent, Nucleus Study & Res Periodont & Implantol NEPPI, Aracatuba, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Aracatuba, SP - Brazil

^[4] Harvard Sch Dent Med, Massachusetts Gen Hosp, Dept Oral & Maxillofacial Surg, Boston, MA - USA

• Document type: Journal article
• Source: CLINICAL ORAL INVESTIGATIONS; v. 26, n. 2 AUG 2021.
• Web of Science Citations: 0

Abstract

Objective To assess the interaction between chemotherapy and normal tissues is critical to assure quality of life during and after the treatment of cancer. This study evaluated the influence of cisplatin (CIS) and 5-fluorouracil (5-FU) over the periimplant tissues around osseointegrated titanium implants in animals previously exposed to nicotine. Materials and methods One hundred twenty male rats were divided into two groups, receiving via subcutaneous injection, either physiological saline solution (PSS) (n = 30) or nicotine hemissulfate (NIC) (n = 90) for 30 days prior to implants' placement. One titanium implant (4.0 x 2.2 mm) was installed in each tibia of all animals. PSS and NIC were continued for 30 days after surgery. Five days after cessation, rats were subdivided into three subgroups in accordance with systemic treatments with either PSS, CIS, or 5-FU. Euthanasia was performed at 50, 65, and 95 days post-surgery. Histometric, histopathological, and immunohistochemical analyses were performed. Results NIC-CIS and NIC-5FU presented lower BIC (50, 65, and 95 days) and bone area fraction occupancy (BAFO) (65 and 95 days) than group NIC. Intense inflammatory infiltration, severe tissue breakdown, reduced expression of bone formation biomarkers, and upregulation of TRAP were observed in NIC-CIS and NIC-5FU when compared with group NIC. TRAP expression was significantly higher in NIC-5FU as compared with NIC-CIS at 50 and 95 days. Groups NIC, NIC-CIS, and NIC-5FU presented statistically significant negative impact in all outcome parameters than group PSS. Conclusion CIS and 5-FU severely disrupted the peri-implant tissues around osseointegrated implants in animals previously exposed to nicotine. (AU)

• FAPESP's process: 17/11805-0 - Evaluation of the influence of chemotherapy with cisplatin or 5-Fluorouracil over the peri-implant bone repair process of osseointegrated implants inserted in tibiae of rats modified or not by nicotine
• Grantee: Henrique Rinaldi Matheus
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 14/11427-8 - Influence of chemotherapy drugs in the evolution of experimental periodontitis in rats
• Grantee: Juliano Milanezi de Almeida
• Support Opportunities: Regular Research Grants