Effect of TiF4/NaF and chitosan solutions on the development of enamel caries under a microcosm biofilm model

Author(s)

Vertuan, Mariele ^[1] ; Machado, Paula Fontana ^[1] ; de Souza, Beatriz Martines ^[1] ; Braga, Aline Silva ^[1] ; Magalhaes, Ana Carolina ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Al Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

• Document type: Journal article
• Source: Journal of Dentistry; v. 111, AUG 2021.
• Web of Science Citations: 0

Abstract

Objectives: To evaluate the effect of experimental solutions containing TiF4/NaF and chitosan on bacterial species and on enamel caries prevention. Methods: Microcosm biofilm was produced from human saliva mixed with McBain saliva (0.2% sucrose) on bovine enamel for five days, under 5% CO2 and 37 degrees C. From the second day until the end, the treatments were applied (1 x 60 s/day): (1) NaF (500 ppm F-, positive control); (2) TiF4 and NaF (TiF4: 190 ppm Ti4+ and 300 ppm F-; NaF: 190 ppm F-); (3) similar to 2 plus 0.5% chitosan (Ch 500 mPas, 75% deacetylation); (4) phosphate buffer solution (negative control); and (5) 0.5% chitosan (Ch 500 mPas, 75% deacetylation). CFU counting was performed for total microorganism, total streptococcus, total lactobacillus and Streptococcus mutans. Enamel demineralization was measured by transverse microradiography-TMR. The data were compared using ANOVA/ Tukey or Kruskal-Wallis/Dunn tests (p<.050). Results: No differences were found between the treatments with respect to CFU counting (ANOVA, p>.050). Enamel treated with TiF4/NaF plus chitosan solution presented the lowest demineralization compared to the negative control and pure chitosan solution. On the other hand, this experimental solution did not significantly differ from TiF4/NaF and NaF solutions, being all of them able to significantly reduce mineral loss (50-74%), but only TiF4/NaF plus chitosan reduced lesion depth (55%) compared to the negative control (p = .001). Conclusion: TiF4/NaF plus chitosan solution had no antimicrobial effect, but it was able to reduce enamel caries development in 79% compared to control under this model. Clinical significance: This study showed that TiF4/NaF plus chitosan solution had no antimicrobial effect, but it was able to reduce enamel caries development under a microcosm biofilm model. (AU)

• FAPESP's process: 17/27056-7 - Abrasive potential of whitening toothpastes on eroded dentin: an in vitro study
• Grantee: Paula Fontana Machado
• Support Opportunities: Scholarships in Brazil - Scientific Initiation