Effects of growth hormone-releasing hormone agonistic analog MR-409 on insulin-secreting cells under cyclopiazonic acid-induced endoplasmic reticulum stress

Author(s):Show less -

Rodrigues-dos-Santos, Karina ^{[1, 2]} ; Soares, Gabriela M. ^{[1, 2]} ; Guimaraes, Dimitrius S. P. S. F. ^{[1, 2]} ; Araujo, Thiago R. ^{[1, 2]} ; Vettorazzi, Jean F. ^{[3, 2]} ; Zangerolamo, Lucas ^{[1, 2]} ; Marconato-Junior, Emilio ^{[1, 2]} ; Cai, Renzhi ^{[4, 5, 6, 7, 8]} ; Sha, Wei ^{[4, 5, 6, 7, 8]} ; Schally, V, Andrew ; Boschero, Antonio C. ^{[1, 2]} ; Barbosa, Helena C. L. ^{[1, 2]}

Affiliation

^[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Struct & Funct Biol, POB 6109, BR-13083865 Campinas - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Inst Biol, Obes & Comorbid Res Ctr, Campinas, SP - Brazil

^[3] UNIVEL, Educ Union Cascavel, Cascavel, Parana - Brazil

^[4] V, Vet Affairs Med Ctr, 1201 NW 16th St, Res Serv 151, Room 2A103C, Miami, FL 33125 - USA

^[5] V, Univ Miami, Miller Sch Med, Dept Pathol, Div Hematol Oncol, 1600 NW 10th Ave 1140, Miami, FL 33136 - USA

^[6] V, Univ Miami, Miller Sch Med, Dept Pathol, Div Endocrinol, 1600 NW 10th Ave 1140, Miami, FL 33136 - USA

^[7] V, Univ Miami, Miller Sch Med, Dept Med, Div Hematol Oncol, 1600 NW 10th Ave 1140, Miami, FL 33136 - USA

^[8] V, Univ Miami, Miller Sch Med, Dept Med, Div Endocrinol, 1600 NW 10th Ave 1140, Miami, FL 33136 - USA

• Document type: Journal article
• Source: Molecular and Cellular Endocrinology; v. 535, SEP 15 2021.
• Web of Science Citations: 0

Abstract

The endoplasmic reticulum (ER) stress is one of the mechanisms related to decreased insulin secretion and beta cell death, contributing to the progress of type 2 diabetes mellitus (T2D). Thus, investigating agents that can influence this process would help prevent the development of T2D. Recently, the growth-hormone-releasing hormone (GHRH) action has been demonstrated in INS-1E cells, in which it increases cell proliferation and insulin secretion. As the effects of GHRH and its agonists have not been fully elucidated in the beta cell, we proposed to investigate them by evaluating the role of the GHRH agonist, MR-409, in cells under ER stress. Our results show that the agonist was unable to ameliorate or prevent ER stress. However, cells exposed to the agonist showed less oxidative stress and greater survival even under ER stress. The mechanisms by which GHRH agonist, MR-409, leads to these outcomes require further investigation. (AU)

• FAPESP's process: 15/12611-0 - Molecular mechanisms involved in pancreatic beta cell disfunction and dead in diabetes mellitus: strategies for the inhibition of these processes and restoration of the insular mass
• Grantee: Antonio Carlos Boschiero
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/00665-6 - Role of GHRH on INS-1E beta-cells: structural proteomic analysis and potential for recovery of endoplasmic reticulum stress
• Grantee: Karina Rodrigues dos Santos
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 18/06363-1 - Therapeutic potential of GHRH and metformin on pancreatic beta cell function against endoplasmic reticulum stress and type 2 diabetes mellitus progress
• Grantee: Helena Cristina de Lima Barbosa
• Support Opportunities: Regular Research Grants