The Heparan Sulfate Binding Peptide in Tumor Progression of Triple-Negative Breast Cancer

Author(s)

Melo, Carina Mucciolo ^{[1, 2]} ; Wang, Huawei ^[3] ; Fujimura, Ken ^[3] ; Strnadel, Jan ^[4] ; Meneghetti, Maria Cecilia Zorel ^[2] ; Nader, Helena Bonciani ^[2] ; Klemke, Richard L. ^[3] ; Pinhal, Maria Aparecida Silva ^{[1, 2]}

Affiliation

^[1] Fac Med ABC, Dept Biochem, Santo Andre, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Biochem Mol Biol, Sao Paulo - Brazil

^[3] Univ Calif San Diego, Sch Med, Dept Pathol, La Jolla, CA 92093 - USA

^[4] Comenius Univ, Jessenius Fac Med Martin, Biomed Ctr Martin, Dept Mol Med, Martin - Slovakia

• Document type: Journal article
• Source: FRONTIERS IN ONCOLOGY; v. 11, AUG 4 2021.
• Web of Science Citations: 0

Abstract

Angiogenesis is the formation of new vessels from pre-existing vasculature. The heparan sulfate chains from endothelial cell proteoglycans interact with the major angiogenic factors, regulating blood vessels ` formation. Since the FDA ` s first approval, anti-angiogenic therapy has shown tumor progression inhibition and increased patient survival. Previous work in our group has selected an HS-binding peptide using a phage display system. Therefore, we investigated the effect of the selected peptide in angiogenesis and tumor progression. The HS-binding peptide showed a higher affinity for heparin N-sulfated. The HS-binding peptide was able to inhibit the proliferation of human endothelial umbilical cord cells (HUVEC) by modulation of FGF-2. It was verified a significant decrease in the tube formation of human endothelial cells and capillary formation of mice aorta treated with HS-binding peptide. HS-binding peptide also inhibited the formation of sub-intestinal blood vessels in zebrafish embryos. Additionally, in zebrafish embryos, the tumor size decreased after treatment with HS-binding peptide. (AU)

• FAPESP's process: 19/03024-4 - Effect of antioxidants on modulation of lipoprotein lipase by alteration of heparan sulfate proteoglycans in endothelial cells
• Grantee: Carina Mucciolo Melo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/01357-8 - Heparan sulfate, heparanase and HER2 binding peptides with potential antineoplastic effect
• Grantee: Maria Aparecida da Silva Pinhal
• Support Opportunities: Regular Research Grants