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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A mechanistic approach towards the formation of bityrosine in proteins by ionizing radiation-GYG model peptide

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Author(s):
Sowinski, Sebastian [1] ; Varca, Gustavo H. C. [1, 2] ; Kadlubowski, Slawomir [1] ; Lugao, Ademar B. [2] ; Ulanski, Piotr [1]
Total Authors: 5
Affiliation:
[1] Lodz Univ Technol, Fac Chem, Inst Appl Radiat Chem IARC, Wroblewskiego 15, PL-93590 Lodz - Poland
[2] Inst Pesquisas Energet & Nucl IPEN CNEN SP, Av Prof Lineu Prestes 2242, Cidade Univ, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Radiation Physics and Chemistry; v. 188, NOV 2021.
Web of Science Citations: 0
Abstract

One of the methods of protein crosslinking used in the synthesis of protein-based nanoparticles is the formation of bityrosine bridges, which may be achieved by the recombination of phenoxyl-type radicals in irradiated protein solutions. Radiation-induced formation of phenoxyl radicals in tyrosine is promoted in presence of H+. In this work, kinetics of this process and the influence of pH were studied by pulse radiolysis measurements of a model peptide H-Gly-Tyr-Gly-OH (GYG), which may help to solve questions raised for bigger, more complex systems in comparable conditions. The main route of phenoxyl radicals formation consists of the addition reaction of hydroxyl radical to the phenol ring on the tyrosine side-chain and proton catalyzed water molecule elimination. A similar effect was observed in studies of tripeptide solutions containing phosphate buffer. The presented data include time-resolved optical spectroscopy of transient species formed under pulse electron beam irradiation and a comparison of the kinetics of the phenoxyl radicals formation in samples at various pH and in presence of phosphate buffer. The postulated mechanism and obtained values of rate constants of the formation and decay of transient species were additionally checked by simple probabilistic simulations. (AU)

FAPESP's process: 10/10935-9 - Development of a nanostructured hydrogel containing a papain and cyclodextrin complex
Grantee:Gustavo Henrique Costa Varca
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)