| Full text | |
| Author(s): |
Silva, Nadine C.
[1]
;
Alvarez, Angela M.
[1, 2]
;
DeOcesano-Pereira, Carlos
[2]
;
Fortes-Dias, Consuelo L.
[3]
;
Moreira, Vanessa
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Fed Sao Paulo, Escola Paulista Med, Pharmacol Dept, BR-04044020 Sao Paulo, SP - Brazil
[2] Butantan Inst, CENTD, BR-05503900 Sao Paulo, SP - Brazil
[3] Fundacao Ezequiel Dias FUNED, BR-30510010 Belo Horizonte, MG - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | International Journal of Biological Macromolecules; v. 187, p. 603-613, SEP 30 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Although crotoxin B (CB) is a well-established catalytically active secretory phospholipase A(2) group IIA (sPLA(2)-IIA) myotoxin, we investigated its potential stimulatory effect on myogenesis with the involvement of prostaglandins (PGs) produced by cyclooxygenase (COX)-1 and -2 pathways. Myoblast C2C12 were cultured in proliferation or commitment protocols and incubated with CB followed by lumiracoxib (selective COX-2 inhibitor) or valeryl salicylate (selective COX-1 inhibitor) and subjected to analysis of PG release, cell proliferation and activation of myogenic regulatory factors (MRFs). Our data showed that CB in non-cytotoxic concentrations induces an increase of COX-2 protein expression and stimulates the activity of both COX isoforms to produce PGE(2), PGD(2) and 15d-PGJ(2). CB induced an increase in the proliferation of C2C12 myoblast cells dependent on PGs from both COX-1 and COX-2 pathways. In addition, CB stimulated the activity of Pax7, MyoD, Myf5 and myogenin in proliferated cells. Otherwise, CB increased myogenin activity but not MyoD in committed cells. Our findings evidence the role of COX-1- and COX-2-derived PGs in modulating CB-induced activation of MRFs. This study contributes to the knowledge that CB promote early myogenic events via regulatory mechanisms on PG-dependent COX pathways, showing new concepts about the effect of sPLA(2)-IIA in skeletal muscle repair. (AU) | |
| FAPESP's process: | 17/15107-6 - Role of prostaglandins in the proliferation and differentiation of C2C12 muscle cells, induced by a phospholipase A2 isolated from snake venom |
| Grantee: | Nadine Cardoso Silva |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 15/25437-8 - Characterization of the release profile and the regulatory activity of eicosanoids in the degeneration and regeneration of skeletal muscle following injury |
| Grantee: | Vanessa Moreira |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/10937-3 - Satellite cells: Effects of lipocalin, hemolin and derived peptides on proliferation and differentiation of quiescent skeletal muscle cells. |
| Grantee: | Angela María Alvarez Gómez |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |