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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Catalytically active phospholipase A(2) myotoxin from Crotalus durissus terrificus induces proliferation and differentiation of myoblasts dependent on prostaglandins produced by both COX-1 and COX-2 pathways

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Silva, Nadine C. [1] ; Alvarez, Angela M. [1, 2] ; DeOcesano-Pereira, Carlos [2] ; Fortes-Dias, Consuelo L. [3] ; Moreira, Vanessa [1]
Total Authors: 5
[1] Univ Fed Sao Paulo, Escola Paulista Med, Pharmacol Dept, BR-04044020 Sao Paulo, SP - Brazil
[2] Butantan Inst, CENTD, BR-05503900 Sao Paulo, SP - Brazil
[3] Fundacao Ezequiel Dias FUNED, BR-30510010 Belo Horizonte, MG - Brazil
Total Affiliations: 3
Document type: Journal article
Source: International Journal of Biological Macromolecules; v. 187, p. 603-613, SEP 30 2021.
Web of Science Citations: 0

Although crotoxin B (CB) is a well-established catalytically active secretory phospholipase A(2) group IIA (sPLA(2)-IIA) myotoxin, we investigated its potential stimulatory effect on myogenesis with the involvement of prostaglandins (PGs) produced by cyclooxygenase (COX)-1 and -2 pathways. Myoblast C2C12 were cultured in proliferation or commitment protocols and incubated with CB followed by lumiracoxib (selective COX-2 inhibitor) or valeryl salicylate (selective COX-1 inhibitor) and subjected to analysis of PG release, cell proliferation and activation of myogenic regulatory factors (MRFs). Our data showed that CB in non-cytotoxic concentrations induces an increase of COX-2 protein expression and stimulates the activity of both COX isoforms to produce PGE(2), PGD(2) and 15d-PGJ(2). CB induced an increase in the proliferation of C2C12 myoblast cells dependent on PGs from both COX-1 and COX-2 pathways. In addition, CB stimulated the activity of Pax7, MyoD, Myf5 and myogenin in proliferated cells. Otherwise, CB increased myogenin activity but not MyoD in committed cells. Our findings evidence the role of COX-1- and COX-2-derived PGs in modulating CB-induced activation of MRFs. This study contributes to the knowledge that CB promote early myogenic events via regulatory mechanisms on PG-dependent COX pathways, showing new concepts about the effect of sPLA(2)-IIA in skeletal muscle repair. (AU)

FAPESP's process: 17/15107-6 - Role of prostaglandins in the proliferation and differentiation of C2C12 muscle cells, induced by a phospholipase A2 isolated from snake venom
Grantee:Nadine Cardoso Silva
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 15/25437-8 - Characterization of the release profile and the regulatory activity of eicosanoids in the degeneration and regeneration of skeletal muscle following injury
Grantee:Vanessa Moreira
Support Opportunities: Regular Research Grants
FAPESP's process: 18/10937-3 - Satellite cells: effects of lipocalin, hemolin and derived peptides on proliferation and differentiation of quiescent skeletal muscle cells
Grantee:Angela María Alvarez Gómez
Support Opportunities: Scholarships in Brazil - Post-Doctoral