(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)
Catalytically active phospholipase A(2) myotoxin from Crotalus durissus terrificus induces proliferation and differentiation of myoblasts dependent on prostaglandins produced by both COX-1 and COX-2 pathways
Silva, Nadine C.
Alvarez, Angela M.
Fortes-Dias, Consuelo L.
Total Authors: 5
 Univ Fed Sao Paulo, Escola Paulista Med, Pharmacol Dept, BR-04044020 Sao Paulo, SP - Brazil
 Butantan Inst, CENTD, BR-05503900 Sao Paulo, SP - Brazil
 Fundacao Ezequiel Dias FUNED, BR-30510010 Belo Horizonte, MG - Brazil
Total Affiliations: 3
International Journal of Biological Macromolecules;
SEP 30 2021.
Web of Science Citations:
Although crotoxin B (CB) is a well-established catalytically active secretory phospholipase A(2) group IIA (sPLA(2)-IIA) myotoxin, we investigated its potential stimulatory effect on myogenesis with the involvement of prostaglandins (PGs) produced by cyclooxygenase (COX)-1 and -2 pathways. Myoblast C2C12 were cultured in proliferation or commitment protocols and incubated with CB followed by lumiracoxib (selective COX-2 inhibitor) or valeryl salicylate (selective COX-1 inhibitor) and subjected to analysis of PG release, cell proliferation and activation of myogenic regulatory factors (MRFs). Our data showed that CB in non-cytotoxic concentrations induces an increase of COX-2 protein expression and stimulates the activity of both COX isoforms to produce PGE(2), PGD(2) and 15d-PGJ(2). CB induced an increase in the proliferation of C2C12 myoblast cells dependent on PGs from both COX-1 and COX-2 pathways. In addition, CB stimulated the activity of Pax7, MyoD, Myf5 and myogenin in proliferated cells. Otherwise, CB increased myogenin activity but not MyoD in committed cells. Our findings evidence the role of COX-1- and COX-2-derived PGs in modulating CB-induced activation of MRFs. This study contributes to the knowledge that CB promote early myogenic events via regulatory mechanisms on PG-dependent COX pathways, showing new concepts about the effect of sPLA(2)-IIA in skeletal muscle repair. (AU)