Burkholderia from Fungus Gardens of Fungus-Growing Ants Produces Antifungals That Inhibit the Specialized Parasite Escovopsis

Author(s)

Francoeur, Charlotte B. ^{[1, 2]} ; May, Daniel S. ^[1] ; Thairu, Margaret W. ^[1] ; Hoang, Don Q. ^{[1, 2]} ; Panthofer, Olivia ^[1] ; Bugni, Tim S. ^[3] ; Pupo, Monica T. ^[4] ; Clardy, Jon ^[5] ; Pinto-Tomas, Adrian A. ^{[6, 7, 8]} ; Currie, Cameron R. ^{[1, 2]}

Affiliation

^[1] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 - USA

^[2] Univ Wisconsin, Dept Energy, Great Lakes Bioenergy Res Ctr, Madison, WI 53706 - USA

^[3] Univ Wisconsin, Sch Pharm, Pharmaceut Sci Div, 425 N Charter St, Madison, WI 53706 - USA

^[4] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Ribeirao Preto - Brazil

^[5] Harvard Med Sch, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 - USA

^[6] Univ Costa Rica, Ctr Invest Estruct Microscop, San Jose - Costa Rica

^[7] Univ Costa Rica, Fac Med, Dept Bioquim, San Jose - Costa Rica

^[8] Univ Costa Rica, Ctr Invest Biol Celular & Mol, San Jose - Costa Rica

• Document type: Journal article
• Source: Applied and Environmental Microbiology; v. 87, n. 14 JUL 2021.
• Web of Science Citations: 0

Abstract

Within animal-associated microbiomes, the functional roles of specific microbial taxa are often uncharacterized. Here, we use the fungus-growing ant system, a model for microbial symbiosis, to determine the potential defensive roles of key bacterial taxa present in the ants' fungus gardens. Fungus gardens serve as an external digestive system for the ants, with mutualistic fungi in the genus Leucoagaricus converting the plant substrate into energy for the ants. The fungus garden is host to specialized parasitic fungi in the genus Escovopsis. Here, we examine the potential role of Burkholderia spp. that occur within ant fungus gardens in inhibiting Escovopsis. We isolated members of the bacterial genera Burkholderia and Paraburkholderia from 50% of the 52 colonies sampled, indicating that members of the family Burkholderiaceae are common inhabitants in the fungus gardens of a diverse range of fungus-growing ant genera. Using antimicrobial inhibition bioassays, we found that 28 out of 32 isolates inhibited at least one Escovopsis strain with a zone of inhibition greater than 1 cm. Genomic assessment of fungus garden-associated Burkholderiaceae indicated that isolates with strong inhibition all belonged to the genus Burkhoideria and contained biosynthetic gene clusters that encoded the production of two antifungals: burkholdine1213 and pyrrolnitrin. Organic extracts of cultured isolates confirmed that these compounds are responsible for antifungal activities that inhibit Escovopsis but, at equivalent concentrations, not Leucoagaricus spp. Overall, these new findings, combined with previous evidence, suggest that members of the fungus garden microbiome play an important role in maintaining the health and function of fungus-growing ant colonies. IMPORTANCE Many organisms partner with microbes to defend themselves against parasites and pathogens. Fungus-growing ants must protect Leucoagaricus spp., the fungal mutualist that provides sustenance for the ants, from a specialized fungal parasite, Escovopsis. The ants take multiple approaches, including weeding their fungus gardens to remove Escovopsis spores, as well as harboring Pseudonocardia spp., bacteria that produce antifungals that inhibit Escovopsis. In addition, a genus of bacteria commonly found in fungus gardens, Burkholderia, is known to produce secondary metabolites that inhibit Escovopsis spp. In this study, we isolated Burkholderia spp. from fungus-growing ants, assessed the isolates' ability to inhibit Escovopsis spp., and identified two compounds responsible for inhibition. Our findings suggest that Burkholderia spp. are often found in fungus gardens, adding another possible mechanism within the fungus-growing ant system to suppress the growth of the specialized parasite Escovopsis. (AU)

• FAPESP's process: 13/50954-0 - Novel therapeutic agents from the bacterial symbionts of Brazilian invertebrates
• Grantee: Mônica Tallarico Pupo
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants