| Full text | |
| Author(s): |
Almeida, Cristina Ferreira
[1]
;
Amaral, Cristina
[1]
;
Augusto, V, Tiago
;
Correia-da-Silva, Georgina
[2]
;
de Andrade, Camila Marques
[3]
;
Torqueti, Maria Regina
[3]
;
Teixeira, Natercia
[2]
Total Authors: 7
|
| Affiliation: | [1] Univ Porto, Fac Pharm, Dept Biol Sci, UCIBIO REQUIMTE, Lab Biochem, Rua Jorge Viterbo Ferreira 228, P-4050313 Porto - Portugal
[2] Augusto, Tiago, V, Univ Porto, Fac Pharm, Dept Biol Sci, UCIBIO REQUIMTE, Lab Biochem, Rua Jorge Viterbo Ferreira 228, P-4050313 Porto - Portugal
[3] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Lab Clin Cytol, Ribeirao Preto - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Toxicon; v. 200, p. 69-77, SEP 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Estrogen receptor-positive (ER+) breast cancer is the most diagnosed subtype of breast cancer. Currently, ammatase inhibitors (AIs) are used as first-line treatment option in this type of tumors, however they cause several side effects, which is why new therapeutic approaches are demanding. The South American rattlesnake Crotalus durissus terrificus produces a venom enriched in several bioactive substances, like phospholipases A(2) (PLA(2)). One of those is cmtoxin, a beta-neurotoxin, that has already been reported for its anti-cancer properties in different cancers. Recently, its clinical interest has emerged and, in fact, a clinical trial in patients with advanced cancer is underway. Considering this, in this work, we studied the biological mechanisms behind the anti-cancer effects of crotoxin B (CTX) in an ER+ aromatase-overexpressing breast cancer cell line (MCF-7aro cells). Results revealed that CTX impairs MCF-7aro cells growth, through a cell cycle arrest at G(2)/M phase, inhibition of ERK1/2 pathway and by apoptosis through activation of caspase-8. In addition, it can be considered a safe natural compound as did not affect non-cancerous cells and only showed anti-growth effects in breast cancer cells. Therefore, this study represents an important landmark to better understand the effects and mechanisms of action of crotoxin in ER+ breast cancer. (AU) | |
| FAPESP's process: | 17/25532-6 - Evaluation of crotoxin antitumoral activity in breast cancer: apoptose, autophagy and oxidative stress |
| Grantee: | Maria Regina Torqueti |
| Support Opportunities: | Regular Research Grants |