| Full text | |
| Author(s): |
Cortez Marcolino, Luciana Maria
[1]
;
Correia Pereira, Andre Henrique
[1]
;
Pinto, Juliana Guerra
[1]
;
Ariel Mamone, Leandro
[2, 3]
;
Strixino, Juliana Ferreira
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Vale Paraiba, Photobiol Appl Hlth, Av Shishima Hifumi 2911, Sao Jose Dos Campos, SP - Brazil
[2] Consejo Nacl Invest Cient & Tecn, Ctr Invest Porfirinas & Porfirias CIPYP, Cordoba 2351 1er Subsuelo, Buenos Aires - Argentina
[3] Univ Buenos Aires, Hosp Clin Jose de San Martin, Cordoba 2351 1er Subsuelo, Buenos Aires - Argentina
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Photodiagnosis and Photodynamic Therapy; v. 35, SEP 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Leishmaniasis is a zoonotic disease, regarded by WHO as a public health problem that has presented a significant increase in the recent years. Conventional treatment is toxic and leads to serious side effects. Photodynamic therapy has been studied as a treatment to cutaneous leishmaniasis. This study aimed to evaluate the cell viability, morphological changes, type of cell death, production of reactive oxygen species, and changes in the mitochondrial membrane and DNA fragmentation in Leishmania braziliensis and Leishmania major promastigotes. Confocal microscopy was used to quantify the fluorescence emitted by JC-1, Annexin V, and propidium iodide reagents. The trypan blue exclusion test was used to evaluate the viability of the cells, the mitochondrial activity was verified with MTT, and the morphological changes were analyzed for SEM and DNA damage using the comet assay. PDT using curcumin at 500, 125, and 31,25 mu g/mL decreased the viability of the parasites and induced changes in the mitochondrial membrane potential. The production of reactive oxygen species was dose dependent and was observed only in the groups submitted to PDT. DNA damage was also observed in the parasite cells. The morphology of the cells was affected mainly at the highest curcumin concentration, resulting in rounded cells with a shortened flagellum. When the type of cell death was analyzed, the prevalence of apoptosis was noted. The results support the use of curcumin as photosensitizer in PDT against Leishmania promastigotes in the treatment for cutaneous leishmaniasis. (AU) | |
| FAPESP's process: | 13/07276-1 - CEPOF - Optics and Photonic Research Center |
| Grantee: | Vanderlei Salvador Bagnato |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |