| Full text | |
| Author(s): Show less - |
Marcelino da Silva, Elaine Zayas
[1]
;
de Campos Fraga-Silva, Thais Fernanda
[2]
;
Yuan, Yao
[3]
;
Alves, Marcia Gaiao
[1]
;
Publio, Gabriel Azevedo
[4]
;
da Fonseca, Carol Kobori
[1]
;
Kodama, Marcio Hideki
[1]
;
Vieira, Gabriel Viliod
[1]
;
Candido, Marina Ferreira
[1]
;
Ramos Innocentini, Lara Maria Alencar
[5]
;
Miranda, Mateus Goncalves
[1]
;
da Silva, Alfredo Ribeiro
[6]
;
Alves-Filho, Jose Carlos
[4]
;
Deperon Bonato, Vania Luiza
[2]
;
Iglesias-Bartolome, Ramiro
[3]
;
Sales, Katiuchia Uzzun
[1]
Total Authors: 16
|
| Affiliation: | [1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell & Mol Biol & Pathogen Bioagents, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, Basic & Appl Immunol Program, BR-14049900 Ribeirao Preto, SP - Brazil
[3] NCI, Lab Cellular & Mol Biol, Ctr Canc Res, NIH, Bethesda, MD 20892 - USA
[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, BR-14049900 Ribeirao Preto, SP - Brazil
[5] Univ Sao Paulo, Clin Hosp Ribeirao Preto Med Sch, Dent & Stomatol Div, Ophthalmol Otolaryngol & Head & Neck Surg Dept, BR-14049900 Ribeirao Preto, SP - Brazil
[6] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pathol & Legal Med, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | CANCERS; v. 13, n. 17 SEP 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Simple Summary Head and neck squamous cell carcinomas (HNSCC) are among the most common cancers worldwide. In contrast to the advances in prevention and treatment of other types of cancer, the five-year survival rate for HNSCC is only about 50% and it has not changed for the past 50 years. This poor prognosis is mainly due to a shortage of suitable markers for early detection, delayed diagnosis and/or referral, and ineffectiveness of chemotherapy. The aim of this study was to explore the inhibitory role of LEKTI in matriptase-dependent squamous cell carcinogenesis and to investigate additional players operating in this pathway. We found that Kallikrein-5 is necessary for PAR-2-mediated IL-8 release, YAP1-TAZ/TEAD activation, and matriptase-mediated oral squamous cell carcinoma migration. This knowledge can contribute for the development of future targeted therapy in HNSCC. Head and neck squamous cell carcinoma remains challenging to treat with no improvement in survival rates over the past 50 years. Thus, there is an urgent need to discover more reliable therapeutic targets and biomarkers for HNSCC. Matriptase, a type-II transmembrane serine protease, induces malignant transformation in epithelial stem cells through proteolytic activation of pro-HGF and PAR-2, triggering PI3K-AKT-mTOR and NFKB signaling. The serine protease inhibitor lympho-epithelial Kazal-type-related inhibitor (LEKTI) inhibits the matriptase-driven proteolytic pathway, directly blocking kallikreins in epithelial differentiation. Hence, we hypothesized LEKTI could inhibit matriptase-dependent squamous cell carcinogenesis, thus implicating kallikreins in this process. Double-transgenic mice with simultaneous expression of matriptase and LEKTI under the keratin-5 promoter showed a prominent rescue of K5-Matriptase(+/0) premalignant phenotype. Notably, in DMBA-induced SCC, heterotopic co-expression of LEKTI and matriptase delayed matriptase-driven tumor incidence and progression. Co-expression of LEKTI reverted altered Kallikrein-5 expression observed in the skin of K5-Matriptase(+/0) mice, indicating that matriptase-dependent proteolytic pathway inhibition by LEKTI occurs through kallikreins. Moreover, we showed that Kallikrein-5 is necessary for PAR-2-mediated IL-8 release, YAP1-TAZ/TEAD activation, and matriptase-mediated oral squamous cell carcinoma migration. Collectively, our data identify a third signaling pathway for matriptase-dependent carcinogenesis in vivo. These findings are critical for the identification of more reliable biomarkers and effective therapeutic targets in Head and Neck cancer. (AU) | |
| FAPESP's process: | 17/24730-9 - Generation and phenotyping of K5-Kallikrein 5 transgenic mice |
| Grantee: | Elaine Zayas Marcelino da Silva |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 16/13228-8 - Genetic study of LEKTI superexpression in HNSCC mouse model |
| Grantee: | Elaine Zayas Marcelino da Silva |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 14/06316-2 - Genetic epistasis analysis of matriptase-depedent proteolytic pathway in head and neck cancer |
| Grantee: | Katiuchia Uzzun Sales |
| Support Opportunities: | Research Grants - Young Investigators Grants |