Messastrum gracile (Chlorophyceae) growth using sugarcane molasses-based macrophyte extract culture media

Author(s)

Tedesque, Mayara Galatti ^[1] ; Scardoeli-Truzzi, Bruno ^[1] ; Sipauba-Tavares, Lucia Helena ^[1]

Affiliation

^[1] Univ Estadual Paulista, Aquaculture Ctr, Lab Limnol & Plankton Prod, BR-14884900 Jaboticabal, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF APPLIED PHYCOLOGY; v. 33, n. 5, p. 2745-2754, OCT 2021.
• Web of Science Citations: 0

Abstract

The current study investigated whether untreated and pretreated molasses is suitable for Messastrum gracile growth and the influence of extracts of macrophyte species used as culture media. There was a difference in nutrient composition of the macrophyte culture media with high concentrations of P and N (60 mg L-1) in Salvinia auriculata extract culture medium. When exposed to sugarcane molasses, the availability of these nutrients in the culture medium did not directly affect microalgae growth, since the highest growth rate (k = 0.22) and cell density (204 x 10(5) cells mL(-1)) were observed in Lemna minor culture medium, where N and P concentrations were below 0.3 mg L-1. In macrophyte culture media, total inorganic nitrogen concentrations were higher than total phosphorous. Lipid contents were different under both mixotrophic conditions; they were higher under untreated molasses, except S. auriculata culture medium; however, protein contents under two conditions were lower than 24% biomass dry weight. Low-cost production with annual costs of US\$ 961.62 and annual biomass production of US\$ 5.20 per tonne dry biomass was observed. Current analysis shows that sugarcane molasses (under untreated and pretreated) is not a good alternative to improve and maintain M. gracile, although the opposite occurred with macrophyte culture media that have low cost and high nutrient concentrations enabling the cultivation of this microalga in laboratory. (AU)

• FAPESP's process: 14/24697-3 - Use of biotechnological macrophytes in aquaculture
• Grantee: Lúcia Helena Sipaúba Tavares
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/11001-9 - Use of in natura sugar cane molecule and hydrolyzed cultivation of microalga Ankistrodesmus gracilis (Reinsch) Korshikov in alternative means
• Grantee: Mayara Galatti Tedesque
• Support Opportunities: Scholarships in Brazil - Master