MicroRNA roles in regeneration: Multiple lessons from zebrafish

Author(s)

Ribeiro, Amanda Oliveira ^[1] ; de Oliveira, Arthur Casulli ^[1] ; Costa, Juliana Mara ^[1] ; Nachtigall, Pedro Gabriel ^{[1, 2]} ; Herkenhoff, Marcos Edgar ^{[1, 3]} ; Campos, Vinicius Farias ^[4] ; Delella, Flavia Karina ^[5] ; Pinhal, Danillo ^[1]

Affiliation

^[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Lab Genom Evolucao Mol LGEM, Dept Ciencias Quim & Biol, Botucatu, SP - Brazil

^[2] Inst Butantan, CeTICS, Lab Especial Toxicol Aplicada LETA, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Dept Tecnol Bioquim Farmaceut, Fac Ciencias Farmaceut, Sao Paulo, SP - Brazil

^[4] Univ Fed Pelotas, Lab Genom Estrutural, Programa Posgrad Biotecnol, Ctr Desenvolvimento Tecnol, Pelotas, RS - Brazil

^[5] Univ Estadual Paulista Julio de Mesquita Filho UN, Dept Biol Estrutural & Func, Inst Biociencias, Botucatu, SP - Brazil

• Document type: Review article
• Source: DEVELOPMENTAL DYNAMICS; SEP 2021.
• Web of Science Citations: 0

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs with pivotal roles in the control of gene expression. By comparing the miRNA profiles of uninjured vs. regenerating tissues and structures, several studies have found that miRNAs are potentially involved in the regenerative process. By inducing miRNA overexpression or inhibition, elegant experiments have directed regenerative responses validating relevant miRNA-to-target interactions. The zebrafish (Danio rerio) has been the epicenter of regenerative research because of its exceptional capability to self-repair damaged tissues and body structures. In this review, we discuss recent discoveries that have improved our understanding of the impact of gene regulation mediated by miRNAs in the context of the regeneration of fins, heart, retina, and nervous tissue in zebrafish. We compiled what is known about the miRNA control of regeneration in these tissues and investigated the links among up-regulated and down-regulated miRNAs, their putative or validated targets, and the regenerative process. Finally, we briefly discuss the forthcoming prospects, highlighting directions and the potential for further development of this field. (AU)

• FAPESP's process: 14/03062-0 - Molecular characterization of heterotic hybrid from Red Stirling and Chitralada lines of Nile Tilapia (Oreochromis niloticus)
• Grantee: Marcos Edgar Herkenhoff
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/06864-7 - Functional analysis of microRNAs in cardiac development of fish species by RNA-Seq and reverse genetics
• Grantee: Pedro Gabriel Nachtigall
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/14348-2 - Functional analysis of microRNAs on cellular dedifferentiation during tissue regeneration in the zebrafish model
• Grantee: Amanda de Oliveira Ribeiro
• Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training
• FAPESP's process: 18/05484-0 - Functional analysis of microRNAs on cellular dedifferentiation during tissue regeneration in the zebrafish model
• Grantee: Danillo Pinhal
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/15494-5 - Zebrafish genome editing through CRISPR/Cas9 for generation of translational model of tissue regeneration
• Grantee: Danillo Pinhal
• Support Opportunities: Regular Research Grants