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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular collisions or resonance energy transfer in lipid vesicles? A methodology to tackle this question

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Marquezin, Cassia A. [1] ; Teresa Lamy, M. [2] ; de Souza, Eduardo S. [3]
Total Authors: 3
[1] Univ Fed Goias, Inst Fis, CP 131, BR-74001970 Goiania, Go - Brazil
[2] Univ Sao Paulo, Inst Fis, Rua Matao 1371, BR-05508090 Sao Paulo, SP - Brazil
[3] Univ Fed Catalao, Inst Fis, Av Dr Lamartine Pinto de Avelar 1120, BR-75704020 Catalao, Go - Brazil
Total Affiliations: 3
Document type: Journal article
Web of Science Citations: 0

In this work, molecular interactions in a lipid membrane are discussed through fluorescence spectroscopy data, both experimentally and theoretically. In particular, the fluorescence quenching mechanisms between the fluorescent probe 6-dodecanoyl-2-dimethylaminonaphthalene (Laurdan) and the potential drug 2-nitrobenzaldehyde-thiosemicarbazone (2-TSC) were studied, both inserted in a 1,2-dipalmitoyl-snglycero-3-phosphocholine (DMPC) model membrane. The fluorescence intensity and the lifetime of Laurdan decrease dramatically in the presence of 2-TSC, in both gel and fluid phases of the DMPC bilayer. It is shown here how to identify the correct quenching mechanism, by conducting a careful analysis of the fluorescence data. The analysis of the bimolecular constant values obtained through the Stern-Volmer equation, considering the collisional mechanism, made clear the incompatibility of the obtained values with estimated diffusion coefficients for Laurdan and 2-TSC inserted into lipid bilayers. On the other hand, using the Forster's theory of resonance energy transfer (FRET) we obtained results in good agreement with the already known dynamic characteristics of a DMPC bilayer, at its both gel and fluid phases. Through spectroscopy data and computational calculation, Forster distance, energy transfer efficiency and distance distribution were obtained for the donor/acceptor pair Laurdan/2-TSC, at both gel and fluid phases of the bilayer. The distance distribution reflects the occurrence of FRET involving donor/acceptor pairs in the same leaflet of the lipid bilayer and pairs in opposite leaflet, and these results are in good agreement with our previous proposal about the lateral organization and position of Laurdan and 2-TSC molecules in a DMPC bilayer. All these results lead us to conclude that FRET between the donor Laurdan and the acceptor 2-TSC is the mechanism responsible for non-radiative deexcitation of Laurdan. The methodology used here could be extended to other pairs of donor/acceptor molecules, to contribute to the knowledge about their localizations in lipid membranes. (C) 2021 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 21/01593-1 - Multiple physical techniques to structurally characterize biological relevant membranes and its interactions
Grantee:Maria Teresa Moura Lamy
Support Opportunities: Regular Research Grants
FAPESP's process: 18/20162-9 - Investigating the Pathogenesis and Drug Resistance in Microorganisms - Characterization and Control of ATP-Binding Cassette Transporters
Grantee:Andrea Balan Fernandes
Support Opportunities: Regular Research Grants
FAPESP's process: 14/50983-3 - INCT 2014: complex fluids
Grantee:Antonio Martins Figueiredo Neto
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 17/25930-1 - Characterization of lipid dispersions of biological relevance: structures and interactions
Grantee:Maria Teresa Moura Lamy
Support Opportunities: Regular Research Grants