Investigating the Pathogenesis and Drug Resistance in Microorganisms - Characterization and Control of ATP-Binding Cassette Transporters

• Grant number: 18/20162-9
• Support Opportunities: Regular Research Grants
• Duration: February 01, 2019 - April 30, 2021
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Andrea Balan Fernandes
• Grantee: Andrea Balan Fernandes
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

ABC transporters present great medical and pharmaceutical relevance since they are related to the development of the phenomenon of multiple drug resistance (MDR) in the treatment of cancer, bacterial resistance, infectivity and pathogenesis. Although extensively studied in many organisms, there are few functional or structural studies on these transporters in Mycobacterium tuberculosis and Xanthomonas citri pv. citri, two pathogenic bacteria studied by our group. In this project, different approaches will be developed to determine the function of these proteins in the mechanisms of pathogenesis and bacterial resistance, structural studies to understand mechanisms of interaction and ways of inhibiting the transporters. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MEZA, A. N.; CAMBUI, C. C. N.; MORENO, A. C. R.; FESSEL, M. R.; BALAN, A.. Mycobacterium tuberculosis CysA2 is a dual sulfurtransferase with activity against thiosulfate and 3-mercaptopyruvate and interacts with mammalian cells. SCIENTIFIC REPORTS, v. 9, NOV 14 2019. (14/20921-6, 15/14514-1, 16/03196-1, 18/20162-9)

MARQUEZIN, CASSIA A.; TERESA LAMY, M.; DE SOUZA, EDUARDO S.. Molecular collisions or resonance energy transfer in lipid vesicles? A methodology to tackle this question. JOURNAL OF MOLECULAR LIQUIDS, v. 341, NOV 1 2021. (21/01593-1, 18/20162-9, 14/50983-3, 17/25930-1)

VIGNOLI MUNIZ, GABRIEL S.; DE LA TORRE, I, LILIA; DUARTE, EVANDRO L.; LORENZON, ESTEBAN N.; CILLI, EDUARDO M.; BALAN, ANDREA; TERESA LAMY, M.. Interaction of synthetic antimicrobial peptides of the Hylin a1 family with models of eukaryotic structures: Zwitterionic membranes and DNA. BIOCHEMISTRY AND BIOPHYSICS REPORTS, v. 24, DEC 2020. (18/20162-9, 17/25930-1, 14/50983-3)

BARRETO DE OLIVEIRA, MARCELO CASSIO; BALAN, ANDREA. The ATP-Binding Cassette (ABC) Transport Systems in Mycobacterium tuberculosis: Structure, Function, and Possible Targets for Therapeutics. BIOLOGY-BASEL, v. 9, n. 12, DEC 2020. (18/20162-9)

CABARCA, SINDY; DE SOUZA, MAXIMILIA FRAZAO; DE OLIVEIRA, ANDREW ALBERT; VIGNOLI MUNIZ, GABRIEL S.; LAMY, M. TERESA; DOS REIS, CAIO VINICIUS; TAKARADA, JESSICA; EFFER, BRIAN; SOUZA, LUCAS SANTOS; DE LA TORRE, LILIA IRIARTE; et al. Structure of the Mycobacterium tuberculosis cPknF and conformational changes induced in forkhead-associated regulatory domains. CURRENT RESEARCH IN STRUCTURAL BIOLOGY, v. 3, p. 165-178, 2021. (18/20162-9)

MUNIZ, GABRIEL S. VIGNOLI; SOUZA, MARIANA C.; DUARTE, EVANDRO L.; LAMY, M. TERESA. omparing the interaction of the antibiotic levofloxacin with zwitterionic and anionic membranes: Calorimetry, fluorescence, and spin label studie. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v. 1863, n. 7, JUL 1 2021. (18/20162-9, 17/25930-1, 14/50983-3)

DE LA TORRE, I, LILIA; VERGARA MEZA, JOSE G.; CABARCA, SINDY; COSTA-MARTINS, ANDRE G.; BALAN, ANDREA. Comparison of carbohydrate ABC importers from Mycobacterium tuberculosis. BMC Genomics, v. 22, n. 1, NOV 19 2021. (18/20162-9)