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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

SAP30 Gene Is a Probable Regulator of Muscle Hypertrophy in Chickens

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Author(s):
Petry, Bruna [1] ; Moreira, Gabriel Costa Monteiro [1] ; Copola, Aline Goncalves Lio [2] ; de Souza, Marcela Maria [3] ; da Veiga, Fernanda Cristina [1] ; Jorge, Erika Cristina [2] ; de Oliveira Peixoto, Jane [4] ; Ledur, Monica Correa [4] ; Koltes, James E. [3] ; Coutinho, Luiz Lehmann [1]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Luiz de Queiroz Coll Agr ESALQ, Anim Sci Dept, Piracicaba - Brazil
[2] Fed Univ Minas Gerais UFMG, Inst Biol Sci, Dept Morphol, Belo Horizonte, MG - Brazil
[3] Iowa State Univ, Anim Sci Dept, Ames, IA - USA
[4] Embrapa Suinos & Aves, Concordia - Brazil
Total Affiliations: 4
Document type: Journal article
Source: FRONTIERS IN GENETICS; v. 12, SEP 27 2021.
Web of Science Citations: 0
Abstract

Animals with muscle hypertrophy phenotype are targeted by the broiler industry to increase the meat production and the quality of the final product. Studies characterizing the molecular machinery involved with these processes, such as quantitative trait loci studies, have been carried out identifying several candidate genes related to this trait; however, validation studies of these candidate genes in cell culture is scarce. The aim of this study was to evaluate SAP30 as a candidate gene for muscle development and to validate its function in cell culture in vitro. The SAP30 gene was downregulated in C2C12 muscle cell culture using siRNA technology to evaluate its impact on morphometric traits and gene expression by RNA-seq analysis. Modulation of SAP30 expression increased C2C12 myotube area, indicating a role in muscle hypertrophy. RNA-seq analysis identified several upregulated genes annotated in muscle development in treated cells (SAP30-knockdown), corroborating the role of SAP30 gene in muscle development regulation. Here, we provide experimental evidence of the involvement of SAP30 gene as a regulator of muscle cell hypertrophy.</p> (AU)

FAPESP's process: 14/08704-0 - Identification of loci of interest for poultry production
Grantee:Luiz Lehmann Coutinho
Support Opportunities: Research Projects - Thematic Grants