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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Inducible nitric oxide synthase (iNOS) mediates ethanol-induced redox imbalance and upregulation of inflammato cytokines in the kidney

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Author(s):
da Silva, Carla B. P. [1, 2] ; Ceron, Carla S. [3] ; Mendes, Atlante S. [4] ; de Martinis, Bruno S. [5] ; Castro, Michele M. [4] ; Tirapelli, Carlos R. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Escola Enfermagem Ribeirao Preto, DEPCH, Lab Farmacol, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Programa Posgrad Toxicol, Ribeirao Preto, SP - Brazil
[3] Univ Fed Ouro Preto, Dept Ciencias Biol, Ouro Preto, MG - Brazil
[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Ribeirao Preto, SP - Brazil
[5] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ribeirao Preto, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Canadian Journal of Physiology and Pharmacology; v. 99, n. 10, p. 1016-1025, OCT 2021.
Web of Science Citations: 0
Abstract

Overexpression of the inducible isoform of the enzyme nitric oxide synthase (iNOS) has been associated to pathological processes in the kidney. Ethanol consumption induces the renal expression of iNOS; however, the contribution of this enzyme to the deleterious effects of ethanol in the kidney remains elusive. We examined whether iNOS plays a role in the renal dysfunction and oxidative stress induced by ethanol consumption. With this purpose, male C57BL/6 wild-type (WI) or iNOS-deficient (iNOS(-/-)) mice were treated with ethanol (20% WO for 10 weeks. Treatment with ethanol increased the expression of Nox4 as well as the concentration of thiobarbituric acid reactive substances and the levels of tumor necrosis factor a in the renal cortex of ANT but not iNOS(-/-) mice. Augmented serum levels of creatinine and increased systolic blood pressure were found in ANT and iNOS(-/-) mice treated with ethanol. WT mice treated with ethanol showed increased production of reactive oxygen species and myeloperoxidase activity, but these responses were attenuated in iNOS(-/-) mice. We concluded that iNOS played a role in ethanol-induced oxidative stress and pro-inflammatory cytokine production in the kidney. These are mechanisms that may contribute to the renal toxicity induced by ethanol. (AU)

FAPESP's process: 16/17623-9 - MECHANISMS UNDERLYING THE RENAL TOXICITY INDUCED BY CHRONIC ETHANOL CONSUMPTION: THE ROLE OF THE INDUCIBLE NITRIC OXIDE SYNTHASE (iNOS)
Grantee:Carla Brigagão Pacheco da Silva
Support Opportunities: Scholarships in Brazil - Doctorate