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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Investigation of canine visceral leishmaniasis in a non-endemic area in Brazil and the comparison of serological and molecular diagnostic tests

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Anaiá da Paixão Sevá ; Ana Pérola Drulla Brandão [2] ; Silvia Neri Godoy [3] ; Rodrigo Martins Soares [4] ; Helio Langoni [5] ; Bruna Cristine Rodrigues [6] ; Mariana Zanchetta e Gava [7] ; Paula Ferraz de Camargo Zanotto [8] ; Tatiana Jimenez-Villegas [9] ; Roberto Hiramoto [10] ; Fernando Ferreira [11]
Total Authors: 11
Document type: Journal article
Source: Revista da Sociedade Brasileira de Medicina Tropical; v. 54, 2021-09-06.

Abstract INTRODUCTION: Visceral leishmaniasis (VL) is an important zoonosis in Brazil. Previous identification of parasitized dogs can also help prevent the disease in humans, even in non-endemic areas of the country. The Brazilian Ministry of Health recommends diagnosis in dogs using a DPP® (rapid test) as a screening test and an immunoenzymatic assay (ELISA) as a confirmatory test (DPP®+ELISA), and culling infected dogs as a legal control measure. However, the accuracy of these serological tests has been questioned. METHODS: VL in dogs was investigated in a non-endemic area of the São Paulo state for three consecutive years, and the performances of different diagnostic tests were compared. RESULTS: A total of 331 dog samples were collected in 2015, 373 in 2016, and 347 in 2017. The seroprevalence by DPP®+ELISA was 3.3, 3.2, and 0.3%, respectively, and by indirect immunofluorescence assay (IFA), it was 3.0, 5.6, and 5.5%, respectively. ELISA confirmed 18.4% of DPP® positive samples. The concordance between the IFA and DPP® was 83.9%. The concordance between IFA and DPP®+ELISA was 92.9%. A molecular diagnostic test (PCR) was performed in 63.2% of the seropositive samples, all of which were negative. CONCLUSIONS: In non-endemic areas, diagnostic tests in dogs should be carefully evaluated to avoid false results. (AU)

FAPESP's process: 15/21966-6 - Epidemiological investigation of domestic dogs that inhabit the surrounding Carlos Botelho State Park - São Paulo, Brazil: its implication on animal, environmental and public health
Grantee:Anaiá da Paixão Sevá
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/21954-8 - Free-ranging domestic dogs and cats in Carlos Botelho State Park: what are the impacts on wild carnivores and implications for public health?
Grantee:Fernando Ferreira
Support type: Regular Research Grants