Contamination gradient affects differently carbonic anhydrase activity of mollusks depending on their feeding habits

Author(s)

Yokota Harayashiki, Cyntia Ayumi ^[1] ; Sadauskas-Henrique, Helen ^[2] ; de Souza-Bastos, Luciana Rodrigues ^[3] ; Gouveia, Nayara ^[1] ; Luna, Ana Julya ^[1] ; Ostrensky, Antonio ^[4] ; Castro, Italo Braga ^[1]

Affiliation

^[1] Univ Fed Sao Paulo IMAR UNIFESP, Inst Mar, Santos, SP - Brazil

^[2] Univ Santa Cecilia Unisanta, Lab Organismos Marinhos & Costeiros LABOMAC, Santos, SP - Brazil

^[3] Inst Tecnol Desenvolvimento LACTEC, Lab Toxicol & Avaliacao Ambiental, Rodovia BR 116, Km 98, N 8813 Jardim Amer, BR-81531980 Curitiba, Parana - Brazil

^[4] Univ Fed Parana, Dept Zootecnia, Grp Integrad Aquicultura & Estudos Ambientais GIA, Rua Funcionarios 1540, BR-80035050 Curitiba, Parana - Brazil

• Document type: Journal article
• Source: ECOTOXICOLOGY; v. 31, n. 1 NOV 2021.
• Web of Science Citations: 0

Abstract

Aquatic organisms that inhabit coastal areas are often exposed to several contaminants. It is known that the bioaccumulation of contaminants can be amplified according to the species feeding habits and contaminant properties. As a consequence, species can experience different effects to contaminant exposure even if they inhabit the same area. The present study aimed to investigate the activities of carbonic anhydrase (CA), Ca2+-ATPase, and Mg2+-ATPase in different tissues (soft tissue, mantle, and gill) of three mollusk species (Lottia subrugosa, Stramonita brasiliensis, and Crassostrea brasiliana) with different feeding habits (herbivore, carnivore, and filter-feeder, respectively) which were sampled within a known contamination gradient at Santos Estuarine System (Southeastern Brazil). From the three enzymes tested, only CA was affected by the presence of contaminants within the contamination gradient evaluated. In general, the CA activity from the three species were lower in contaminated sites when compared to the reference site. The contrasting CA activity response observed in S. brasiliensis compared to L. subrugosa and C. brasiliana could be related to the tissue-specificity of this enzyme activity and species feeding habits (filter-feeders can accumulate more contaminants than herbivores and even carnivores). Results indicated that C. brasiliana mantle is the most suitable tissue for the use of CA analysis as a biomarker. (AU)

• FAPESP's process: 18/08015-0 - Shell structural alterations and biochemical responses in limpets as biomarkers of environmental contamination
• Grantee: Cyntia Ayumi Yokota Harayashiki
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 19/23310-1 - Shell alterations in Stramonita brasiliensis as possible biomarkers of contamination in coastal areas
• Grantee: Ana Julya Luna Andrade de Oliveira
• Support type: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 15/25063-0 - Shell alterations in Lottia subrugosa as putative biomarker for multi-impacted coastal areas
• Grantee: Ítalo Braga de Castro
• Support type: Regular Research Grants