The 6-hydroxydopamine Rat Model of Parkinson's Disease

Author(s)

Guimaraes, Rayanne Poletti ^[1] ; Ribeiro, Danilo Leandro ^[1] ; dos Santos, Keila Bariotto ^[1] ; Godoy, Livea Dornela ^[1] ; Correa, Mirella Rosine ^[1] ; Padovan-Neto, Fernando Eduardo ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Psychol, Sao Paulo - Brazil

• Document type: Journal article
• Source: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS; n. 176 OCT 2021.
• Web of Science Citations: 0

Abstract

Motor symptoms of Parkinson's disease (PD)-bradykinesia, akinesia, and tremor at rest-are consequences of the neurodegeneration of dopaminergic neurons in the substantia nigra pars compacta (SNc) and dopaminergic striatal deficit. Animal models have been widely used to simulate human pathology in the laboratory. Rodents are the most used animal models for PD due to their ease of handling and maintenance. Moreover, the anatomy and molecular, cellular, and pharmacological mechanisms of PD are similar in rodents and humans. The infusion of the neurotoxin, 6hydroxydopamine (6-OHDA), into a medial forebrain bundle (MFB) of rats reproduces the severe destruction of dopaminergic neurons and simulates PD symptoms. This protocol demonstrates how to perform the unilateral microinjection of 6-OHDA in the MFB in a rat model of PD and shows the motor deficits induced by 6-OHDA and predicted dopaminergic lesions through the stepping test. The 6-OHDA causes significant impairment in the number of steps performed with the contralateral forelimb. (AU)

• FAPESP's process: 17/00003-0 - Role of neuronal nitric oxide synthase and phosphodiesterase 10A in striatal medium spiny neuron activity during L-Dopa-induced dyskinesia
• Grantee: Fernando Eduardo Padovan Neto
• Support Opportunities: Research Grants - Young Investigators Grants