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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Promising Natural Compounds against Flavivirus Proteases: Citrus Flavonoids Hesperetin and Hesperidin

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Author(s):
Eberle, Raphael J. [1, 2] ; Olivier, Danilo S. [3] ; Amaral, Marcos S. [4] ; Willbold, Dieter [1, 5, 2] ; Arni, Raghuvir K. [6] ; Coronado, Monika A. [2]
Total Authors: 6
Affiliation:
[1] Heinrich Heine Univ Dusseldorf, Inst Phys Biol, Univ Str, D-40225 Dusseldorf - Germany
[2] Forschungszentrum Julich, Inst Biol Informat Proc IBI 7 Struct Biochem, D-52428 Julich - Germany
[3] Fed Univ Tocantins, Integrated Sci Ctr, BR-77824838 Araguaina - Brazil
[4] Univ Fed Mato Grosso do Sul, Inst Phys, BR-79070900 Campo Grande, MS - Brazil
[5] Forchungszentrum Julich, JuStruct Julich Ctr Struct Biol, D-52428 Julich - Germany
[6] Univ Estadual Paulista UNESP, Multiuser Ctr Biomol Innovat, Dept Phys, BR-15054000 Sao Jose Do Rio Preto - Brazil
Total Affiliations: 6
Document type: Journal article
Source: PLANTS-BASEL; v. 10, n. 10 OCT 2021.
Web of Science Citations: 0
Abstract

Ubiquitous in citrus plants, Hesperidin and Hesperetin flavanones possess several biological functions, including antiviral activity. Arbovirus infections pose an ever-increasing threat to global healthcare systems. Among the severe arboviral infections currently known are those caused by members of the Flavivirus genus, for example, Dengue Virus-DENV, Yellow Fever Virus-YFV, and West Nile Virus-WNV. In this study, we characterize the inhibitory effect of Hesperidin and Hesperetin against DENV2, YFV, and WNV NS2B/NS3 proteases. We report the noncompetitive inhibition of the NS2B/NS3(pro) by the two bioflavonoids with half maximal inhibitory concentration (IC50) values < 5 mu M for HST and < 70 mu M for HSD. The determined dissociation constants (K-D) of both flavonoids is significantly below the threshold value of 30 mu M. Our findings demonstrate that a new generation of anti-flavivirus drugs could be developed based on selective optimization of both molecules.</p> (AU)

FAPESP's process: 18/07572-3 - Exploring the non-structural protease nsP2 from Chikungunya and Mayaro viruses: structures and inhibition.
Grantee:Raphael Josef Eberle
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 19/05614-3 - Identification of bioactive molecules which inhibit both Chikungunya and Mayaro virus activities.
Grantee:Raphael Josef Eberle
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 18/12659-0 - Mechanisms and Molecular Interactions of Bioactive Molecules with Protein NS3 from Zika Virus - De novo drug design-
Grantee:Monika Aparecida Coronado
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 16/12904-0 - Mechanism and Molecular Interactions of Bioactive molecules with NS3 protease from Zika virus.
Grantee:Monika Aparecida Coronado
Support Opportunities: Scholarships in Brazil - Post-Doctoral