| Full text | |
| Author(s): |
Britto-Junior, Jose
[1]
;
Ximenes, Luiz
[1]
;
Ribeiro, Andre
[1]
;
Fregonesi, Adriano
[1]
;
Campos, Rafael
[2, 3]
;
Kiguti, Luiz Ricardo de Almeida
[4]
;
Monica, Fabiola Z.
[1]
;
Antunes, Edson
[1]
;
De Nucci, Gilberto
[5, 1, 2, 4]
Total Authors: 9
|
| Affiliation: | [1] State Univ Campinas UNICAMP, Fac Med Sci, Dept Pharmacol, Campinas, SP - Brazil
[2] Fed Univ Ceara UFC, Drug Res & Dev Ctr, Clin Pharmacol Unit, Fortaleza, CE - Brazil
[3] Ceara State Univ UECE, Super Inst Biomed Sci, Fortaleza - Brazil
[4] Metropolitan Univ Santos UNIMES, Santos - Brazil
[5] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | European Journal of Pharmacology; v. 911, NOV 15 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
6-nitrodopamine (6-ND) is released from human umbilical cord vessels and modulates vascular reactivity by acting as a dopamine antagonist. Here we investigate whether 6-ND is released by the rat isolated vas deferens and its effect on this tissue. Dopamine, noradrenaline, adrenaline and 6-ND levels were quantified in rat isolated vas deferens by LC-MS-MS. Electric-field stimulation (EFS) and concentration-response curves to 6-ND, noradrenaline, dopamine and adrenaline were performed in the absence and in the presence (30 min) of LNAME, SCH-23390, haloperidol, PG-01037, sonepiprazole, desipramine, clomipramine, amitriptyline, cyclobenzaprine, carbamazepine, maprotiline, paroxetine, oxcarbazepine and ketanserin in the rat isolated epididymal vas deferens (RIEVD). Basal releases of 6-ND and noradrenaline were detected from the rat isolated vas deferens. 6-ND release was reduced by tissue incubation with L-NAME and from the vas deferens obtained from L-NAME-treated rats. SCH-23390 caused leftward shifts on concentration-response curves to 6-ND without affecting dopamine- or EFS-induced RIEVD contractions. Haloperidol, PG-01037 and sonepiprazole caused significant rightward shifts on concentration-response curves to dopamine but had no effect on either the 6-ND or EFS-induced RIEVD contractions. The tricyclic compounds desipramine, clomipramine, amitriptyline, cyclobenzaprine and carbamazepine induced rightward shifts on 6-ND concentration-response curve but did not reduce the noradrenaline, dopamine and adrenaline contractile responses. They also reduced the EFS-induced RIEVD contractions in control but not in tissues obtained from L-NAME-treated animals. Maprotiline, oxcarbazepine, paroxetine and ketanserin had no effect in either 6-ND or EFS-induced RIEVD contractions. Thus, 6-ND modulates RIEVD contractility, and desipramine, clomipramine, amitriptyline, cyclobenzaprine and carbamazepine act as selective 6-ND receptor antagonists. (AU) | |
| FAPESP's process: | 17/15175-1 - Modulation of soluble guanylate cyclase and the intracellular levels of cyclic nucleotides in the lower urinary tract and prostate |
| Grantee: | Edson Antunes |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 19/16805-4 - Evaluation of the pathophysiological role of endothelial catecholamines |
| Grantee: | Gilberto de Nucci |
| Support Opportunities: | Research Projects - Thematic Grants |