Physicochemical and biological properties of a biostimulating membrane (BBio) for pulp capping

Author(s)

Neto, Natalino Lourenco ^[1] ; Vitor, Luciana Lourenco Ribeiro ^[2] ; da Costa, Silgia Aparecida ^[3] ; da Costa, Sirlene Maria ^[3] ; Cruvinel, Thiago ^[1] ; Oliveira, Thais Marchini ^[1] ; Oliveira, Rodrigo Cardoso ^[4] ; Machado, Maria Aparecida Andrade Moreira ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Discipline Pediat Dent, Bauru, SP - Brazil

^[2] Unisagrado, Sch Dent, Dept Pediat Dent, Bauru, SP - Brazil

^[3] Univ Sao Paulo, Sch Arts Sci & Humanities, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci Discipline Biochem, Bauru, SP - Brazil

• Document type: Journal article
• Source: Materials Letters; v. 308, n. B FEB 1 2022.
• Web of Science Citations: 0

Abstract

The aim of this study was to develop and characterize a pulp capping multilayer membrane with chitosan, regarding physicochemical properties of radiopacity, pH, calcium release, and biological properties of cytocompatibility. Human pulp fibroblasts (HPF) exposed to membrane solutions were subjected to MTT assay for viability analysis. All developed and tested membranes showed alkaline pH, satisfactory calcium release, and radiopacity higher than 3 mm Al. The cytocompatibility assay demonstrated the highest scores of viability at 48 h, without statistical difference between groups overtime. Although these results were compatible with properties of adequate pulp capping materials, further in vivo and clinical studies are needed to determine the suitability of these membranes to be applied in human teeth. (AU)

• FAPESP's process: 13/07836-7 - Analysis and development of a dentin/pulpar biostimulating bandage (BBio)
• Grantee: Natalino Lourenço Neto
• Support Opportunities: Scholarships in Brazil - Post-Doctoral