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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

ffects of different stimulators of cGMP synthesis on lipid content in bovine oocytes matured in vitr

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Author(s):
Schefer, Leticia [1] ; Lancelloti Schwarz, Katia Regina [1] ; Paschoal, Daniela Martins [1] ; de Castro, Fernanda Cavallari [1] ; Fernandes, Hugo [1] ; Botigelli, Ramon Cesar [1] ; Verde Leal, Claudia Lima [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Med Vet, Pirassununga, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: ANIMAL REPRODUCTION; v. 18, n. 4 2021.
Web of Science Citations: 0
Abstract

Bovine oocytes and blastocysts produced in vitro are frequently of lower quality and less cryotolerant than those produced in vivo, and greater accumulation of lipids in the cytoplasm has been pointed out as one of the reasons. In human adipocytes cGMP signaling through the activation of PKG appears to be involved in lipid metabolism, and components of this pathway have been detected in bovine cumulus-oocyte complexes (COCs). The aim of this study was to investigate the influence of this pathway on the lipid content in oocytes and expression of PLIN2 (a lipid metabolism-related gene) in cumulus cells. C005 were matured in vitro for 24 h with different stimulators of cGMP synthesis. The activation of soluble guanylyl cyclase (sGC) by Protoporphyrin IX reduced lipid content (22.7 FI) compared to control oocytes (36.45 FI; P <0.05). Stimulation of membrane guanylyl cyclase (mGC) with natriuretic peptides precursors A and C (NPPA and NPPC) had no effect (36.5 FI; P>0.05). When the PKG inhibitor KT5823 was associated with Protoporphyrin IX, its effect was reversed and lipid contents increased (52.71 FI; P<0.05). None of the stimulators of cGMP synthesis affected the expression of PLIN2 in cumulus cells. In conclusion, stimulation of sGC for cGMP synthesis promotes lipolytic activities in bovine oocytes matured in vitro and such effect is mediated by PKG. However, such effect may vary depending on the stimulus received and/or which synthesis enzyme was activated, as stimulation of mGC had no effects. (AU)

FAPESP's process: 15/20379-0 - Studies regarding melatonin on the function and quality of bovine cumulus-oocyte complexes
Grantee:Cláudia Lima Verde Leal
Support Opportunities: Regular Research Grants
FAPESP's process: 15/19888-7 - Semi-quantitative assessment of the lipid content of bovine oocytes in vitro matured under the influence of the cGMP pathway
Grantee:Letícia Schefer
Support Opportunities: Scholarships in Brazil - Scientific Initiation