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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response

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Author(s):
Biagi Jr, Carlos Alberto Oliveira ; Cury, Sarah Santiloni [1] ; Alves, Cleidson Padua [2] ; Rabhi, Nabil [3] ; Silva Jr, Wilson Araujo ; Farmer, Stephen R. [3] ; Carvalho, Robson Francisco [1] ; Batista Jr, Miguel Luiz
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, BR-18618689 Botucatu - Brazil
[2] Univ Cologne, Med Fac, Dept Translat Gen, D-50923 Cologne - Germany
[3] Boston Univ, Sch Med, Dept Biochem, Boston, MA 02215 - USA
Total Affiliations: 3
Document type: Journal article
Source: CELLS; v. 10, n. 11 NOV 2021.
Web of Science Citations: 0
Abstract

Adipose tissue has been classified based on its morphology and function as white, brown, or beige/brite. It plays an essential role as a regulator of systemic metabolism through paracrine and endocrine signals. Recently, multiple adipocyte subtypes have been revealed using RNA sequencing technology, going beyond simply defined morphology but also by their cellular origin, adaptation to metabolic stress, and plasticity. Here, we performed an in-depth analysis of publicly available single-nuclei RNAseq from adipose tissue and utilized a workflow template to characterize adipocyte plasticity, heterogeneity, and secretome profiles. The reanalyzed dataset led to the identification of different subtypes of adipocytes including three subpopulations of thermogenic adipocytes, and provided a characterization of distinct transcriptional profiles along the adipocyte trajectory under thermogenic challenges. This study provides a useful resource for further investigations regarding mechanisms related to adipocyte plasticity and trans-differentiation. (AU)

FAPESP's process: 18/20905-1 - TLR4 as crossroad of inflammation and energy metabolism: Role of adipose tissue in cancer cachexia
Grantee:Miguel Luiz Batista Junior
Support Opportunities: Scholarships abroad - Research