ell differentiation events in pre-implantation mouse and bovine embryo

Author(s)

Carreiro, Leticia Escobar ^[1] ; dos Santos, Gabriel Siqueira ^[1] ; Luedke, Felipe Eduardo ^[1] ; Goissis, Marcelo Demarchi ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Reprod Anim, Fac Med Vet & Zootecnia, Sao Paulo, SP - Brazil

• Document type: Review article
• Source: ANIMAL REPRODUCTION; v. 18, n. 4 2021.
• Web of Science Citations: 0

Abstract

Early mammal embryogenesis starts with oocyte fertilization, giving rise to the zygote. The events that the newly formed zygote surpasses are crucial to the embryo developmental success. Shortly after activation of its genome, cells of the embryo segregate into the inner cell mass (ICM) or the trophectoderm (TE). The first will give rise to the embryo while the latter will become the placenta. This first segregation involves cellular and molecular processes that include cell polarity linked to intracellular pathway activation, which will regulate the transcription of trophectoderm-related genes. Then, cells of the ICM undergo the second event of mammalian cell differentiation, which consists of the separation between epiblast (EPI) and hypoblast or primitive endoderm (PrE). This second segregation involves paracrine signaling, leading to differential expression of key genes that will dictate the fate of the cell. Although these processes are described in detail in the mouse, recent studies suggest that the bovine embryo could also be an interesting model for early development, since there are differences to the mouse and similarities with early human embryogenesis. In this review, we gathered the main data available in the literature upon bovine and mouse early development events, suggesting that both models should be analyzed and studied in a complementary way, to better model early events occurring in human development. (AU)

• FAPESP's process: 19/22649-5 - Functional evaluation of NANOG gene in segregation of cell lineages in bovine embryos
• Grantee: Letícia Escobar Carreiro
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 19/21303-8 - Effect of inhibition of fibroblast growth factor receptors in the differentiation of primitive endoderm in bovine embryos produced in vitro
• Grantee: Gabriel Siqueira dos Santos
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 19/03014-9 - Insertion of fluorescent reporter gene in Sox2 gene region to study cell differentiation in early bovine embryo development
• Grantee: Felipe Eduardo Luedke
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 17/09576-3 - Studies on cell differentiation mechanisms during early bovine embryo development
• Grantee: Marcelo Demarchi Goissis
• Support Opportunities: Research Grants - Young Investigators Grants