| Full text | |
| Author(s): |
Helaehil, Julia Venturini
[1]
;
Lourenco, Carina Basqueira
[1]
;
Huang, Boyang
[2]
;
Helaehil, Luiza Venturini
[1]
;
de Camargo, Isaque Xavier
[3]
;
Chiarotto, Gabriela Bortolanca
[1]
;
Santamaria-Jr, Milton
;
Bartolo, Paulo
[2, 4]
;
Caetano, Guilherme Ferreira
[5]
Total Authors: 9
|
| Affiliation: | [1] Univ Ctr Herminio Ometto Fdn, Grad Program Biomed Sci, FHO, BR-13607339 Araras, SP - Brazil
[2] Univ Manchester, Dept Mech Aerosp & Civil Engn, Sch Engn, Manchester M13 9PL, Lancs - England
[3] Univ Ctr Herminio Ometto Fdn, Grad Program Orthdnt, FHO, BR-13607339 Araras, SP - Brazil
[4] Nanyang Technol Univ, Singapore Ctr 3D Printing, Sch Mech & Aerosp Engn, Jurong West 639798 - Singapore
[5] Santamaria-Jr, Jr., Milton, Univ Ctr Herminio Ometto Fdn, Grad Program Biomed Sci, FHO, BR-13607339 Araras, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | POLYMERS; v. 14, n. 1 JAN 2022. |
| Web of Science Citations: | 0 |
| Abstract | |
Critical bone defects are a major clinical challenge in reconstructive bone surgery. Polycaprolactone (PCL) mixed with bioceramics, such as hydroxyapatite (HA) and tricalcium phosphate (TCP), create composite scaffolds with improved biological recognition and bioactivity. Electrical stimulation (ES) aims to compensate the compromised endogenous electrical signals and to stimulate cell proliferation and differentiation. We investigated the effects of composite scaffolds (PCL with HA; and PCL with beta-TCP) and the use of ES on critical bone defects in Wistar rats using eight experimental groups: untreated, ES, PCL, PCL/ES, HA, HA/ES, TCP, and TCP/ES. The investigation was based on histomorphometry, immunohistochemistry, and gene expression analysis. The vascular area was greater in the HA/ES group on days 30 and 60. Tissue mineralization was greater in the HA, HA/ES, and TCP groups at day 30, and TCP/ES at day 60. Bmp-2 gene expression was higher in the HA, TCP, and TCP/ES groups at day 30, and in the TCP/ES and PCL/ES groups at day 60. Runx-2, Osterix, and Osteopontin gene expression were also higher in the TCP/ES group at day 60. These results suggest that scaffolds printed with PCL and TCP, when paired with electrical therapy application, improve bone regeneration. (AU) | |
| FAPESP's process: | 18/21167-4 - Use of electroactive ceramic scaffolds and application of low intensity electric current in bone repair |
| Grantee: | Guilherme Ferreira Caetano |
| Support Opportunities: | Regular Research Grants |