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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Proliferation rate and expression of stem cells markers during expansion in primary culture of pulp cells

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Author(s):
Turrioni, Ana Paula [1] ; de Oliveira Neto, Nilson Ferreira [1] ; Xu, Yan [2] ; Morse, Leslie [3] ; de Souza Costa, Carlos Alberto [4] ; Battaglino, Ricardo [3] ; Hebling, Josimeri [5]
Total Authors: 7
Affiliation:
[1] Univ Fed Uberlandia UFU, Sch Dent, Dept Pediat Dent, Uberlandia, MG - Brazil
[2] Forsyth Inst, Dept Mineralized Tissue Biol, Cambridge, MA - USA
[3] Univ Minnesota, Sch Med, Dept Rehabil Med, Minneapolis, MI - USA
[4] Univ Estadual Paulista, UNESP, Sch Dent, Dept Physiol & Pathol, Araraquara, SP - Brazil
[5] Univ Estadual Paulista, UNESP, Sch Dent, Dept Orthodont & Pediat Dent, Araraquara, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Brazilian Oral Research; v. 35, 2021.
Web of Science Citations: 0
Abstract

Abstract: The aim of the present study was to evaluate the proliferation rate and the expression of stem cells markers during expansion in primary culture of dental pulp stem cells (DPSCs), comparing different techniques (explant and enzymatic digestion), subject ages (up to 40 and over 40) and cell passages (#2, #5 and #8). DPSCs were isolated using either the enzymatic digestion (ED) or explant (EX) technique. The number of days needed for the cells to reach confluence was determined. Immunophenotyping was performed by immunofluorescence and flow cytometry analysis using antibodies specific for nestin, vimentin, CD44, CD146, Oct3/4 and CD34. Data were subjected to three-way analysis of variance (n = 6/group). The ANOVA tests were complemented by Tukey's or t-tests (p < 0.05). The variables “donor age” and “technique” were analyzed to define the optimal desirability value using a response optimization. DPSCs presented a high proliferation rate from passages 2 to 5 while cells from passage 8 proliferated at a slower rate. For all markers, no significant difference was observed among passages, irrespective of the technique used or the donor's age. The mean fraction of specific antibodies was 73.7% (± 11.5), 49.0% (± 18.7), 80.1% (± 8.0), 45.2% (± 13.7), 64.7% (± 5.3) and 2.0% (± 1.5) for CD44, OCT, vimentin, nestin, CD146 and CD34, respectively. The highest optimal desirability value was obtained using the ED technique and cells from younger patients (d = 0.92). However, it was concluded that neither the isolation technique nor the donor age or cell passage significantly interfered with the stem cell phenotype and proliferation rate during cell expansion. (AU)

FAPESP's process: 13/17758-3 - Effect of the isolation technique, source of tissue and cell passage on the number and phenotype of stem cells originating from dental pulp
Grantee:Ana Paula Silveira Turrioni
Support Opportunities: Scholarships abroad - Research Internship - Doctorate