eady-to-use room temperature one-pot synthesis of surface-decorated gold nanoparticles with targeting attribute

Author(s)

Ribeiro, Caroline A. S. ^[1] ; Albuquerque, Lindomar J. C. ^{[1, 2]} ; de Castro, Carlos E. ^[1] ; Pereira, Rodrigo M. ^[1] ; Albuquerque, Brunno L. ^[3] ; Pavlova, Ewa ^[4] ; Schluter, Luiza Gabriela ^[5] ; Batista, Bruno L. ^[1] ; Bellettini, Ismael C. ^[5] ; Giacomelli, Fernando C. ^[1]

Affiliation

^[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP - Brazil

^[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Synchrotron Light Lab LNLS, Campinas, SP - Brazil

^[3] Univ Fed Rio Grande Do Sul, Inst Chem, Lab Mol Catalysis, Porto Alegre, RS - Brazil

^[4] Czech Acad Sci, Inst Macromol Chem, Prague - Czech Republic

^[5] Univ Fed Santa Catarina, Dept Ciencias Exatas & Educ, Blumenau - Brazil

• Document type: Journal article
• Source: Journal of Colloid and Interface Science; v. 614, p. 489-501, MAY 15 2022.
• Web of Science Citations: 0

Abstract

Gold nanoparticles (AuNPs) can be used in diagnostic and therapeutic applications. The development of facile and fast synthetic approaches is accordingly desirable towards ready-to-use biomedical materials. We report a practical one-pot method for the synthesis in aqueous media and room temperature of surface-decorated AuNPs with enhanced biological responses. The gold ions could be reduced using only polyethyleneimine (PEI) derivatives containing sugar and-or alkyl chains acting simultaneously as reducing and stabilizing agent, without the aid of any other mediator. The process is possibly potentialized by the presence of the amino groups in the polymer chains which further confer colloidal stability. The kinetics of AuNPs nucleation and growth depends on the chemical nature of the polymer chains. Particularly, the presence of lactose moieties conjugated to the PEI chains conducted to surfacedecorated AuNPs with low cytotoxicity that are remarkably faster uptaken by HepG2 cells. These cells overexpress asialoglycoprotein (ASGP-R), a galactose receptor. These findings may kick off significant advances towards the practical and ready-to-use manufacturing of functionalized AuNPs towards celltargeting since the methodology is applicable for a large variety of other ligands that can be conjugated to the same polymer chains. (C) 2022 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 19/20470-8 - Environmentally-responsive supramolecular polymeric assemblies for the intracellular delivery of therapeutic DNA: towards a toolbox for novel generation of nanopharmaceuticals in ophthalmology
• Grantee: Fernando Carlos Giacomelli
• Support Opportunities: Regular Research Grants