| Full text | |
| Author(s): Show less - |
da Cruz, Adriana Taveira
[1]
;
Hunger, Aline
[2, 3]
;
Machado de Melo, Fabiana Henriques
[4, 1]
;
Monteiro, Ana Carolina
[1, 5]
;
Pare, Genevieve Catherine
[6]
;
Lai, Dulce
[6]
;
Alves-Fernandes, Debora Kristina
[1]
;
Pedroso Ayub, Ana Luisa
[1]
;
Cordero, Esteban Mauricio
[7]
;
da Silveira Filho, Jose Franco
[7]
;
Schneider-Stock, Regine
[5]
;
Strauss, Bryan Eric
[3]
;
Tron, Victor
[6, 7]
;
Jasiulionis, Miriam Galvonas
[1]
Total Authors: 14
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Pharmacol, Escola Paulista Med, Sao Paulo, SP - Brazil
[2] Cristalia, Biotecnol Unidade 1, Rodoviaria SP 147, Itapira, SP - Brazil
[3] Univ Sao Paulo, Fac Med, Ctr Invest Translac Oncol LIM24, Inst Canc Estado Sao Paulo, Lab Vetores Virais, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo, SP - Brazil
[5] Friedrich Alexander Univ, Dept Pathol, Erlangen, Bavaria - Germany
[6] Queens Univ, Dept Pathol & Mol Med, Kingston, ON - Canada
[7] Univ Fed Sao Paulo, Microbiol Immunol & Parasitol Dept, Sao Paulo, SP - Brazil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | Neoplasia; v. 23, n. 8, p. 823-834, AUG 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Deregulation of miRNAs contributes to the development of distinct cancer types, including melanoma, an aggressive form of skin cancer characterized by high metastatic potential and poor prognosis. The expression of a set of 580 miRNAs was investigated in a model of murine melanoma progression, comprising non-metastatic (4C11-) and metastatic melanoma (4C11+) cells. A significant increase in miR-138-5p expression was found in the metastatic 4C11+ melanoma cells compared to 4C11-, which prompted us to investigate its role in melanoma aggressiveness. Functional assays, including anoikis resistance, colony formation, collective migration, serum-deprived growth capacity, as well as in vivo tumor growth and experimental metastasis were performed in 4C11- cells stably overexpressing miR-138-5p. miR-138-5p induced an aggressive phenotype in mouse melanoma cell lines leading to increased proliferation, migration and cell viability under stress conditions. Moreover, by overexpressing miR-138-5p, low-growing and non-metastatic 4C11- cells became highly proliferative and metastatic in vivo, similar to the metastatic 4C11+ cells. Luciferase reporter analysis identified the tumor suppressor Trp53 as a direct target of miR-138-5p. Using data sets from independent melanoma cohorts, miR-138-5p and P53 expression were also found deregulated in human melanoma samples, with their levels negatively and positively correlated with prognosis, respectively. Our data shows that the overexpression of miR-138-5p contributes to melanoma metastasis through the direct suppression of Trp53. (AU) | |
| FAPESP's process: | 12/08776-5 - MicroRNAs involved in melanoma genesis and progression |
| Grantee: | Miriam Galvonas Jasiulionis |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/20775-0 - Non-coding RNAs involved with melanoma genesis and progression |
| Grantee: | Miriam Galvonas Jasiulionis |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 10/18484-6 - Identification of miRNA involved with melanoma genesis and epigenetic regulation of their expression |
| Grantee: | Adriana Taveira da Cruz |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 14/13663-0 - Integrating gene and microRNA expression, methylome and hidroxymethylome data from different phases of melanoma progression. |
| Grantee: | Miriam Galvonas Jasiulionis |
| Support Opportunities: | Regular Research Grants |