verexpression of miR-200b-3p in Menstrual Blood-Derived Mesenchymal Stem Cells from Endometriosis Wome

Author(s):Show less -

de Oliveira, Rafael Zucco ^[1] ; Buono, Fabiana de Oliveira ^[1] ; Lagazzi Cressoni, Ana Clara ^[1] ; Correa Penariol, Leticia Bruna ^[1] ; Padovan, Cristiana Carolina ^[1] ; Tozetti, Patricia Aparecida ^[1] ; Poli-Neto, Omero Benedito ^{[1, 2]} ; Ferriani, Rui Alberto ^{[1, 3]} ; Orellana, Maristela Delgado ^{[4, 5]} ; Rosa-E-Silva, Julio Cesar ^{[1, 2]} ; Meola, Juliana ^{[1, 2, 3]}

Affiliation

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Gynecol & Obstet, Div Human Reprod, 3900 Bandeirantes Ave, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Gynecol & Obstet, Lab Translat Data Sci, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] CNPq, Natl Inst Hormones & Womens Hlth Hormona, BR-90035003 Porto Alegre, RS - Brazil

^[4] Univ Sao Paulo, Ctr Cell Therapy, BR-14051140 Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Reginal Blood Ctr, BR-14051140 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: REPRODUCTIVE SCIENCES; v. 29, n. 3, p. 734-742, MAR 2022.
• Web of Science Citations: 0

Abstract

The key relationship between Sampson's theory and the presence of mesenchymal stem cells in the menstrual flow (MenSCs), as well as the changes in post-transcriptional regulatory processes as actors in the etiopathogenesis of endometriosis, are poorly understood. No study to date has investigated the imbalance of miRNAs in MenSCs related to the disease. Thus, through literature and in silico analyses, we selected four predicted miRNAs as regulators of EGR1, SNAI1, NR4A1, NR4A2, ID1, LAMC3, and FOSB involved in pathways of apoptosis, angiogenesis, response to steroid hormones, migration, differentiation, and cell proliferation. These genes are frequently overexpressed in the endometriosis condition in our group studies. They were the trigger for the miRNAs search. Therefore, a case-control study was conducted with MenSCs of women with and without endometriosis (ten samples per group). Crossing information obtained from the STRING, PubMed, miRPathDB, miRWalk, and DIANA TOOLS databases, we chose to explore the expression of miR-21-5p, miR-100-5p, miR-143-3p, and miR-200b-3p by RT-qPCR. We found an upregulation of the miR-200b-3p in endometriosis MenSCs (P = 0.0207), with a 7.93-fold change (ratio of geometric means) compared to control. Overexpression of miR-200b has been associated with increased cell proliferation, stemness, and accentuated mesenchymal-epithelial transition process in eutopic endometrium of endometriosis. We believe that dysregulated miR-200b-3p may establish primary changes in the MenSCs, thus favoring tissue implantation at the ectopic site. (AU)

• FAPESP's process: 13/22431-3 - Differential transcript profile in mesenchymal stem cells obtained from the menstrual flow (MenMSCs) of women with endometriosis
• Grantee: Juliana Meola Lovato
• Support Opportunities: Research Grants - Young Investigators Grants