| Full text | |
| Author(s): |
Rodrigues e Fonseca, Gabriela
[1]
;
Corral, Marcelo Andreetta
[1]
;
de Paula, Fabiana Martins
[2, 1]
;
Correia Lima Meisel, Dirce Mary
[1]
;
Borges Gryschek, Ronaldo Cesar
[2, 1]
;
Zevallos Lescano, Susana Angelica
[2, 1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Hosp Clin, Fac Med, LIM 06 Imunopatol Esquistossomose, BR-05403000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Med, Inst Med Trop Sao Paulo, Sao Paulo, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Revista do Instituto de Medicina Tropical de São Paulo; v. 64, 2022. |
| Web of Science Citations: | 0 |
| Abstract | |
The Western-blotting technique was applied to identify antigenic fractions of excretory-secretory Toxocara canis antigen recognized by IgG antibodies throughout an experimental infection in mice challenged by different inocula. Mice were inoculated with 5, 50 and 500 embryonated eggs and serum samples were collected 15, 30, 60, 90 and 120 days post-infection. Serum samples were analyzed using an excretory-secretory Toxocara antigen. Antibodies recognized antigenic fractions from 30 to 90 kDa. The protein fraction of 30-35 kDa was the most frequently recognized regardless of the size of inoculum and the stage of infection represented by the different collection times, but the antigenic recognition was more evident in groups infected with 50 and 500 eggs. This study presents an antigenic panel of the excretory secretory antigen of T. canis and suggests that the 30-35 kDa antigenic fraction is a promising marker of the infection and should be further explored in future studies on experimental toxocariasis. (AU) | |
| FAPESP's process: | 14/07345-6 - Use of a polymerase chain reaction technique for Toxocara canis DNA detection in sera of experimentally infected mice |
| Grantee: | Susana Angélica Zevallos Lescano |
| Support Opportunities: | Regular Research Grants |