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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

LA/Hydroxyapatite scaffolds exhibit in vitro immunological inertness and promote robust osteogenic differentiation of human mesenchymal stem cells without osteogenic stimul

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Author(s):
Bernardo, Marcela P. [1, 2] ; da Silva, Bruna C. R. [2] ; Hamouda, I, Ahmed E. ; de Toledo, Marcelo A. S. [3] ; Schalla, Carmen [4] ; Rutten, Stephan [5] ; Goetzke, Roman [6] ; Mattoso, Luiz H. C. [2] ; Zenke, Martin [4] ; Sechi, Antonio [4]
Total Authors: 10
Affiliation:
[1] Rhein Westfal TH Aachen, Inst Biomed Engn, Dept Cell Biol, D-52074 Aachen - Germany
[2] Natl Nanotechnol Lab Agribusiness, Brazilian Agr Res Corp, Embrapa Instrumentat, Rua 15 Novembro, BR-1452 Sao Carlos, SP - Brazil
[3] Rhein Westfal TH Aachen, Dept Hematol Oncol Hemostaseol & Stem Cell Transp, D-52074 Aachen - Germany
[4] Hamouda, Ahmed E., I, Rhein Westfal TH Aachen, Inst Biomed Engn, Dept Cell Biol, D-52074 Aachen - Germany
[5] Rhein Westfal TH Aachen, Electron Microscopy Facil, D-52074 Aachen - Germany
[6] Rhein Westfal TH Aachen, Helmholtz Inst Biomed Engn, Stem Cell Biol & Cellular Engn, D-52074 Aachen - Germany
Total Affiliations: 6
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 12, n. 1 FEB 11 2022.
Web of Science Citations: 0
Abstract

Bone defects stand out as one of the greatest challenges of reconstructive surgery. Fused deposition modelling (FDM) allows for the printing of 3D scaffolds tailored to the morphology and size of bone damage in a patient-specific and high-precision manner. However, FDM still suffers from the lack of materials capable of efficiently supporting osteogenesis. In this study, we developed 3D-printed porous scaffolds composed of polylactic acid/hydroxyapatite (PLA/HA) composites with high ceramic contents (above 20%, w/w) by FDM. The mechanical properties of the PLA/HA scaffolds were compatible with those of trabecular bone. In vitro degradation tests revealed that HA can neutralize the acidification effect caused by PLA degradation, while simultaneously releasing calcium and phosphate ions. Importantly, 3D-printed PLA/HA did not induce the upregulation of activation markers nor the expression of inflammatory cytokines in dendritic cells thus exhibiting no immune-stimulatory properties in vitro. Evaluations using human mesenchymal stem cells (MSC) showed that pure PLA scaffolds exerted an osteoconductive effect, whereas PLA/HA scaffolds efficiently induced osteogenic differentiation of MSC even in the absence of any classical osteogenic stimuli. Our findings indicate that 3D-printed PLA scaffolds loaded with high concentrations of HA are most suitable for future applications in bone tissue engineering. (AU)

FAPESP's process: 18/07860-9 - Development of nanocomposite polymer filaments with anti-inflammatory properties for 3D printing as bone substituent
Grantee:Marcela Piassi Bernardo
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 19/23027-8 - Development of 3D printed scaffolds for bone reconstruction and evaluation of interaction through cellular study
Grantee:Marcela Piassi Bernardo
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor