Effects of different stimulators of cGMP synthesis on lipid content in bovine oocytes matured in vitro

Author(s)

Letícia Schefer ^[1] ; Kátia Regina Lancelloti Schwarz ^[2] ; Daniela Martins Paschoal ^[3] ; Fernanda Cavallari de Castro ^[4] ; Hugo Fernandes ^[5] ; Ramon César Botigelli ^[6] ; Cláudia Lima Verde Leal ^[7]

Affiliation

^[1] Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Medicina Veterinária - Brasil

^[2] Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Medicina Veterinária - Brasil

^[3] Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Medicina Veterinária - Brasil

^[4] Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Medicina Veterinária - Brasil

^[5] Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Medicina Veterinária - Brasil

^[6] Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Medicina Veterinária - Brasil

^[7] Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Medicina Veterinária - Brasil

• Document type: Journal article
• Source: Animal Reproduction; v. 18, n. 4 2021-12-10.

Abstract

Abstract Bovine oocytes and blastocysts produced in vitro are frequently of lower quality and less cryotolerant than those produced in vivo, and greater accumulation of lipids in the cytoplasm has been pointed out as one of the reasons. In human adipocytes cGMP signaling through the activation of PKG appears to be involved in lipid metabolism, and components of this pathway have been detected in bovine cumulus-oocyte complexes (COCs). The aim of this study was to investigate the influence of this pathway on the lipid content in oocytes and expression of PLIN2 (a lipid metabolism-related gene) in cumulus cells. COCs were matured in vitro for 24 h with different stimulators of cGMP synthesis. The activation of soluble guanylyl cyclase (sGC) by Protoporphyrin IX reduced lipid content (22.7 FI) compared to control oocytes (36.45 FI; P <0.05). Stimulation of membrane guanylyl cyclase (mGC) with natriuretic peptides precursors A and C (NPPA and NPPC) had no effect (36.5 FI; P>0.05). When the PKG inhibitor KT5823 was associated with Protoporphyrin IX, its effect was reversed and lipid contents increased (52.71 FI; P<0.05). None of the stimulators of cGMP synthesis affected the expression of PLIN2 in cumulus cells. In conclusion, stimulation of sGC for cGMP synthesis promotes lipolytic activities in bovine oocytes matured in vitro and such effect is mediated by PKG. However, such effect may vary depending on the stimulus received and/or which synthesis enzyme was activated, as stimulation of mGC had no effects. (AU)

• FAPESP's process: 15/20379-0 - Studies regarding melatonin on the function and quality of bovine cumulus-oocyte complexes
• Grantee: Cláudia Lima Verde Leal
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/19888-7 - Semi-quantitative assessment of the lipid content of bovine oocytes in vitro matured under the influence of the cGMP pathway
• Grantee: Letícia Schefer
• Support Opportunities: Scholarships in Brazil - Scientific Initiation