Assessment of redox state and biochemical parameters of salivary glands in rats treated with anti-obesity drug sibutramine hydrochloride

Author(s):Show less -

dos Santos, Damaris Raissa ; Fiais, Gabriela Alice ; Oliveira, Henrique Arnaldo ; Ribas, Tayna Buffulin ; Souza, Rayne Oliveira ; Saori Tsosura, Thais Veronica ; Matsushita, Doris Hissako ; Ervolino, Edilson ; Menegati Dornelles, Rita Cassia ; de Melo Stevanato Nakamune, Ana Claudia ; Chaves-Neto, Antonio Hernandes

• Document type: Journal article
• Source: CLINICAL ORAL INVESTIGATIONS; v. N/A, p. 14-pg., 2022-05-13.

Abstract

Objectives To investigate the effects of anti-obesity drug sibutramine hydrochloride (SB) on redox state and biochemical parameters in the salivary glands. Materials and methods Adult male Wistar rats were randomly divided into the following groups (n = 8 per group): control rats treated with vehicle (C) and rats treated with SB (10 mg/kg/day) by intragastric gavage for 28 days. The parotid (PG) and submandibular (SMG) glands were processed using histomorphometric analysis, and total protein, amylase, mucin, and oxidative damage to lipids were determined by measuring the formation of thiobarbituric acid reactive substances (TBARS), total antioxidant capacity (TAC), uric acid (UA), total glutathione (tGSH), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), and AKT phosphorylation. Results SB decreased the acinar area, and increased the stromal area in PG, while no effect on the morphometric parameters was observed in SMG. SB also increased oxidative damage to lipids (TBARs). The SB group showed lower total protein, amylase, TAC, UA, tGSH, SOD, CAT, and GPx than the C group in PG, while in SMG, SB decreased total protein, mucin, tGSH, SOD, CAT, and GPx. However, increased AKT phosphorylation observed in both salivary glands suggests that SB exerts low-intensity oxidative stress. Conclusions SB impaired enzymatic and non-enzymatic antioxidant defenses in the salivary glands of rats. (AU)

• FAPESP's process: 18/21479-6 - Antioxidant enzymatic, non-enzymatic defenses and lipid oxidative damage in the submandibular glands of rats treated with an anti-obesity drug
• Grantee: Henrique Arnaldo de Oliveira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation