Nitric oxide storage levels modulate vasodilation and the hypotensive effect induced by photobiomodulation using an aluminum gallium arsenide (AlGaAs) diode laser (660 nm)

Author(s)

Buzinari, Tereza Cristina ; de Moraes, Thiago Francisco ; Conceicao-Filho, Julio Cesar ; Carnio, Evelin Capellari ; Almeida-Lopes, Luciana ; Salgado, Helio Cesar ; Rodrigues, Gerson Jhonatan

• Document type: Journal article
• Source: Lasers in Medical Science; v. N/A, p. 10-pg., 2022-04-07.

Abstract

The aim of this study was to evaluate the participation of nitric oxide (NO) in the hypotensive and vasorelaxation effect induced by PBM using an aluminum gallium arsenide (AlGaAs) diode laser (660 nm). Male Wistar rats were treated with the inhibitor of nitric oxide synthase (L-NAME). A red laser (660 nm; 63 J/cm(2); 56 s/point) was applied to the abdominal region at six different points. Thoracic aorta was dissected for vascular reactivity study, and a laser (660 nm; 96 J/cm(2); 56 s) was applied after incubation with the NO donor DETA-NO, PBS, or hydroxicobalamin. Endothelial cells (HUVEC) were treated with DETA-NO or CuSO4, and then, PBM (63 J/cm(2)) was applied, and the nitric oxide was detected. Hypertensive L-NAME rats did not exhibit a decrease in blood pressure after PBM. PBM promoted vasodilation in the aorta isolated from normotensive rats, and less effect in the aorta of L-NAME rats and the addition of the NO donor, DETA-NO, promoted greater vasodilation by PBM in the aorta of L-NAME rats. In endothelial cells, an increase in NO, after PBM, was detected; however, with the addition of CuSO4, which catalyzes the decomposition of NO storage, there was no detection of NO after PBM. The results of this study demonstrate that the hypotensive and vasodilatory effect of PBM with a red laser at 660 nm is modulated by the release of nitric oxide from the storage. (AU)

• FAPESP's process: 18/10588-9 - Characterization of the hypotensive effect by photobiomodulation in different hypertension animal models and pharmacological strategies to enhance this effect
• Grantee: Gerson Jhonatan Rodrigues
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/20549-7 - New insights in cardiovascular regulation under physiological and pathophysiological condition
• Grantee: Helio Cesar Salgado
• Support Opportunities: Research Projects - Thematic Grants