Basic and applied features in the cryopreservation progress of bovine embryos

Author(s)

Valente, Roniele Santana ; Marsico, Thamiris Vieira ; Sudano, Mateus Jose

• Document type: Journal article
• Source: Animal Reproduction Science; v. 239, p. 8-pg., 2022-04-01.

Abstract

The expansion of the use of in vitro production techniques has revolutionized the bovine embryo market. In the last decade, we have seen the number of in vitro produced (IVP) embryos surpass the number of in vivo-derived (IVD) embryos obtained worldwide. Concomitantly, other biotechnologies were also improved, following the global trend. Embryo cryopreservation has received special attention, as it is one of the tools capable of disseminating in vitro production. Currently, two protocols are available: slow freezing and vitrification. Both have advantages and disadvantages regarding their application and, many aspects need to be considered before their use. In this review, we discuss in vitro production market trends, cellular and molecular features involved in embryo response to cryopreservation, and addressed cryo-storage period and embryonic developmental stage on cryosurvival. In addition, we also presented an overview of some aspects that impact the pregnancy rate following transfer of fresh and cryopreserved IVP embryos. (AU)

• FAPESP's process: 18/21496-8 - Pathways for embryo-derived pregnancy
• Grantee: Mateus José Sudano
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 19/17796-9 - Simultaneous biopsy and lipid profile of embryo inner cell mass and trofectoderm
• Grantee: Thamiris Vieira Marsico
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/18415-9 - Role of inflammatory pathways on embryonic competence and cryotolerance
• Grantee: Roniele Santana Valente
• Support Opportunities: Scholarships in Brazil - Doctorate