Tear Film Immunological Profile in Patients with Ocular Graft versus Host Disease

Author(s)

Serapicos, Patricia ; Kim, Cinthia ; Barros, Sabrina Leite ; Mendes Silva Jordao, Isa Maria Bastos ; Hiyane, Meire Ioshie ; de Sousa, Luciene Barbosa ; Zecchin, Victor Gottardello ; Saraiva Camara, Niels Olsen ; de Oliveira, Lauro Augusto

• Document type: Journal article
• Source: OCULAR IMMUNOLOGY AND INFLAMMATION; v. N/A, p. 9-pg., 2022-04-02.

Abstract

Purpose To analyze and compare the tear immunological profile in ocular GVHD (oGVHD) patients with that in non-oGVHD patients and to correlate them with ocular surface parameters based on the International Chronic Ocular GVHD Consensus Group (ICCGVHD) diagnostic criteria. Methods Tear samples from 20 individuals who underwent allo-hematopoietic stem cell transplantation and were grouped according the presence or absence of oGVHD were analyzed using Bio-Plex assay. Results IL-8 and MIP-1 alpha levels were significantly higher in tears from oGVHD patients compared with those in tears from non-oGVHD patients (p<0.001 and p=0.001, respectively). Tear IL-8 levels correlated significantly with OSDI criteria (rho=0.5159, p=0.001), ocular hyperemia (rho=0.469, p=0.002), and corneal staining (rho=0.339, p=0.032), whereas tear Mip-1 alpha levels correlated with OSDI score (rho=0.358, p=0.023). Conclusion We demonstrated higher tear levels of IL-8 and MIP-1 alpha in oGVHD patients and significant correlations between theses cytokines and ocular surface parameters based on the ICCGVHDCG criteria. (AU)

• FAPESP's process: 14/15720-1 - Characterization of tear film inflammatory mediators and of cytologic profile from the ocular surface of patients presenting graft-versus-host-disease
• Grantee: Lauro Augusto de Oliveira
• Support Opportunities: Regular Research Grants