| Full text | |
| Author(s): |
da Silva, Maria Vitoria
;
Santos-Miranda, Artur
;
Joviano-Santos, Julliane, V
;
Souza, Diego Santos
;
Marques, Leisiane Pereira
;
Sarmento, Jaqueline Oliveira
;
Beserra, Samuel Santos
;
Roman-Campos, Danilo
Total Authors: 8
|
| Document type: | Journal article |
| Source: | Clinical Science; v. 136, n. 5, p. 15-pg., 2022-03-01. |
| Abstract | |
3-Cyfluthrin, a class II Pyrethroid, is an insecticide used worldwide in agriculture, horticulture (field and protected crops), viticulture, and domestic applications. 3-Cyfluthrin may impair the function of biological systems; however, little information is available about its poten-tial cardiotoxic effect. Here, we explored the acute toxicity of 3-Cyfluthrin in isolated heart preparations and its cellular basis, using isolated cardiomyocytes. Moreover, 3-Cyfluthrin effects on the sodium current, especially late sodium current (INa-L), were investigated us-ing human embryonic kidney cells (HEK-293) cells transiently expressing human NaV1.5 channels. We report that 3-Cyfluthrin raised INa-L in a dose-dependent manner. 3-Cyfluthrin prolonged the repolarization of the action potential (AP) and triggered oscillations on its duration. Cardiomyocytes contraction and calcium dynamics were disrupted by the pesti-cide with a marked incidence of non-electronic-stimulated contractions. The antiarrhyth-mic drug Ranolazine was able to reverse most of the phenotypes observed in isolated cells. Lastly, ventricular premature beats (VPBs) and long QT intervals were found during 3-Cyfluthrin exposure, and Ranolazine was able to attenuate them. Overall, we demon-strated that 3-Cyfluthrin can cause significant cardiac alterations and Ranolazine ame-liorated the phenotype. Understanding the insecticides' impacts upon electromechanical properties of the heart is important for the development of therapeutic approaches to treat cases of pesticides intoxication. (AU) | |
| FAPESP's process: | 21/05584-7 - Assessment of acute and chronic toxicity of the heart and brain exposed to pesticides found in potable water in Brazil |
| Grantee: | Danilo Roman Campos |
| Support Opportunities: | Research Grants - Young Investigators Grants - Phase 2 |
| FAPESP's process: | 19/18918-0 - Role of nitric oxide in cardiac arrhythmias during Hypothyroidism |
| Grantee: | Diego Santos de Souza |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/20777-3 - Genetic variants of the Nav 1.5 sodium channel and its therapeutic implications. |
| Grantee: | Julliane Vasconcelos Joviano dos Santos |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/22830-9 - Involvement of the Ca2+/ Calmodulin/CAMKII signaling axis in the electrical and contractile dysfunctions of the heart in the chronic phase of Chagas Disease |
| Grantee: | Artur Santos Miranda |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 20/09403-4 - Hydroxychloroquine action on sodium Nav 1.5 from humans: implications for the treatment of COVID-19 |
| Grantee: | Jaqueline Oliveira Sarmento |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 19/21304-4 - Arrhythmogenic mechanisms in right heart diseases |
| Grantee: | Danilo Roman Campos |
| Support Opportunities: | Regular Research Grants |