| Full text | |
| Author(s): |
Gilmara Beatriz Andrade da Silva
[1]
;
Diego Santos Souza
[2]
;
José Evaldo Rodrigues Menezes-Filho
[3]
;
Júlio Alves da Silva-Neto
[4]
;
Jader dos Santos Cruz
[5]
;
Danilo R. Roman-Campos
[6]
;
Lucindo José Quintans-Júnior
[7]
;
Carla Maria Lins de Vasconcelos
[8]
Total Authors: 8
|
| Affiliation: | [1] Universidade Federal de Sergipe - Brasil
[2] Universidade Federal de Sergipe - Brasil
[3] Universidade Federal de Sergipe - Brasil
[4] Universidade Federal de Sergipe - Brasil
[5] Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas - Brasil
[6] Universidade Federal de São Paulo - Brasil
[7] Universidade Federal de Sergipe - Brasil
[8] Universidade Federal de Sergipe - Brasil
Total Affiliations: 8
|
| Document type: | Journal article |
| Source: | Arquivos Brasileiros de Cardiologia; v. 119, n. 2, p. 294-304, 2022-08-05. |
| Abstract | |
Abstract Background: (-)-Carvone is a monoterpene found in essential oils with antioxidant and anti-inflammatory activity. Objective: The aim of this paper was to analyze the antiarrhythmic property of (-)-carvone in the rat heart and its effects on the intracellular Ca2+ signaling. Methods: The effects of (-)-carvone were evaluated on the ventricular (0.5 mM) and atrial contractility (0.01 – 4 mM) and on electrocardiogram (0.5 mM). Fractional shortening, L-type calcium current (ICa,L) and Ca2+ signaling were measured in the isolated cardiomyocyte (0.5 mM). Antiarrhythmic effect was evaluated in arrhythmia model induced by calcium overload (0.5 mM) (n = 5). P < 0.05 was used as the significance level. Results: In the atrium, (-)-carvone evoked negative inotropism that was concentration-dependent (EC50 0.44 ± 0.11 mM) and decreased the positive inotropism evoked by CaCl2 (0.1 to 8.0 mM) or BAY K8644 (5 to 500 nM), an agonist of L-type Ca2+ channel. In isolated heart, (-)-carvone (0.5 mM) promoted reduction of ventricular contractility (73%) and heart rate (46%), increased PRi (30.7%, time from the onset of the P wave until the R wave) and QTc (9.2%, a measure of the depolarization and repolarization of the ventricles) without changing the QRS complex duration. (-)-Carvone decreased the fractional shortening (61%), ICa,L (79%) and Ca2+ intracellular transient (38%). Furthermore, (-)-carvone showed antiarrhythmic action, verified by decrease of the arrhythmia score (85%) and occurrence of ventricular fibrillation. Conclusion: (-)-Carvone decreases Ca2+ entry through L-type Ca2+ channels, reducing the cardiac contractility and intracellular Ca2+, and, therefore, presenting promising antiarrhythmic activity in the rat hearts. (AU) | |
| FAPESP's process: | 19/21304-4 - Arrhythmogenic mechanisms in right heart diseases |
| Grantee: | Danilo Roman Campos |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/18918-0 - Role of nitric oxide in cardiac arrhythmias during Hypothyroidism |
| Grantee: | Diego Santos de Souza |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |