| Full text | |
| Author(s): |
F. Cotinguiba
;
H. M. Debonsi
;
R. V. Silva
[3]
;
R. M. Pioli
;
R. A. Pinto
[5]
;
L. G. Felippe
[6]
;
S. N. López
;
M. J. Kato
[8]
;
M. Furlan
[9]
Total Authors: 9
|
| Document type: | Journal article |
| Source: | Brazilian Journal of Biology; v. 82, 2023-01-13. |
| Abstract | |
Abstract Several Piper species accumulate piperamides as secondary metabolites, and although they have relevant biological importance, many details of their biosynthetic pathways have not yet been described experimentally. Experiments involving enzymatic reactions and labeled precursor feeding were performed using the species Piper tuberculatum and Piper arboreum. The activities of the phenylalanine ammonia lyase (PAL) enzymes, which are involved in the general phenylpropanoid pathway, were monitored by the conversion of the amino acid L-phenylalanine to cinnamic acid. The activity of the 4-hydroxylase (C4H) enzyme was also observed in P. tuberculatum by converting cinnamic acid to p-coumaric acid. L-[UL-14C]-phenylalanine was fed into the leaves of P. tuberculatum and incorporated into piperine (1), 4,5-dihydropiperine (2), fagaramide (4), trans-piplartine (7), and dihydropiplartine (9). In P. arboreum, it was only incorporated into the piperamide 4,5-dihydropiperiline (3). L-[UL-14C]-lysine was successfully incorporated into the 4,5-dihydropiperine piperidine group (2), dihydropyridinone, and trans- (7) and cis-piplartine (8). These data corroborate the proposal of mixed biosynthetic origin of piperamides with the aromatic moiety originating from cinnamic acid (shikimic acid pathway) and key amide construction with amino acids as precursors. (AU) | |
| FAPESP's process: | 14/25705-0 - Metabolic engineering of the ergosterol pathway of Saccharomyces cerevisiae to improve production of the triterpene friedelin |
| Grantee: | Tatiana Maria de Souza Moreira |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 06/50086-5 - Characterization of prenyltransferase of Piper crassinervium and gaudichaudianum (Piperaceae) |
| Grantee: | Silvia Noelí López |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 11/10379-1 - CLONING AND CHARACTERIZATION OF OXIDOSQUALENE CYCLASES FROM Maytenus ilicifolia Mart. ex Reissek |
| Grantee: | Tatiana Maria de Souza Moreira |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 14/03819-3 - Cloning and functional characterization of oxidoesqualene cyclases of Maytenus ilicifolia |
| Grantee: | Cleslei Fernando Zanelli |
| Support Opportunities: | Regular Research Grants |