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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Association between HaeIII and MspI polymorphisms of estrogen receptor alpha gene and mammographic density in post-menopausal women

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Author(s):
Eduardo Henrique de Moura Ramos [1] ; Cláudio Kemp [2] ; Ismael Dale Cotrim Guerreiro da Silva [3]
Total Authors: 3
Affiliation:
[1] Universidade Federal de São Paulo. Escola Paulista de Medicina. Disciplina de Mastologia - Brasil
[2] UNIFESP. Departamento de Ginecologia
[3] Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Ginecologia - Brasil
Total Affiliations: 3
Document type: Journal article
Source: Revista Brasileira de Ginecologia e Obstetrícia; v. 28, n. 10, p. 581-589, 2006-10-00.
Abstract

PURPOSE: To assess the presence of estrogen receptor gene polymorphisms HaeIII and MspI as well as clinical factors, and their possible associations with high mammographic density in post-menopausal women. METHODS: One hundred and fifteen post-menopausal women, not in use of hormonal therapy and without clinical or mammographic lesions were evaluated. Three independent observers have determined the mammographic density pattern based on the ACR-BIRADS® 2003 (two subjective and one objective evaluations - Adobe Photoshop 7.0 software). Oral swabs (Cytobrush) were obtained to extract DNA and the polymerase chain reaction - restriction fragment length polymorphism) was performed to assess the presence of polymorphisms in intron 1 and exon 1 from estrogen receptor gene (HaeIII and MspI). RESULTS: The HaeIII polymorphism was found in 43 (37.4%) of the 115 women, while MspI was found in 96 (83.5%) of them. There was a good agreement among determinations of the three observers with regard to mammographic density. Thirty-four (29.6%) women had dense breasts and eighty-one (70.4%) had non-dense breasts. CONCLUSION: The estrogen receptor gene polymorphism Haelll showed no association with mammographic density (Fisher = 0.712), while the association between estrogen receptor gene polymorphism Mspl and mammographic density was near significance (Fisher = 0.098). The associations among age, parity and body mass index revealed statistical significance. (AU)

FAPESP's process: 03/04533-1 - Study of polymorphisms in genes responsible for biosynthesis, action and metabolization of sexual steroids in estrogen-dependent and menopausal gynecological conditions
Grantee:Ismael Dale Cotrim Guerreiro da Silva
Support Opportunities: Research Projects - Thematic Grants