A New Approach toEx VivoPermeation Studies inIn-SituFilm-Forming Systems

Author(s)

Silva-Alvarez, Amanda F. ; Ferreira, Maira P. ; Vicentini, Fabiana T. M. C. ; Pedrazzi, Vinicius ; de Freitas, Osvaldo

• Document type: Journal article
• Source: AAPS PHARMSCITECH; v. 21, n. 7, p. 7-pg., 2020-09-07.

Abstract

The skin is the largest human organ and an important topical route. Even with some challenges, it is an important ally in medication administration, mainly because it is painless and easy-to-apply. Semisolid formulations are the most used dosage forms for drug administrationviathis delivery route and can be optimized when transformed into a film, favoring on-site maintenance, and promoting drug permeation. However,in situfilm-forming systems are difficult to assess and characterize using Franz-type diffusion cells once this apparatus is ideal to formulations without transition phases. The present study proposed a different method to characterize these formulations and provide complementary data on drug and penetration enhancer behaviors, as close as possible to real application conditions. This characterization method allowed us to analyze drug concentration on three necessary occasions: remaining in the polymer film, stratum corneum using adhesive tape, and skin to check where drugs will have a desirable effect. As a proof-of-concept, the proposedex vivopermeation method was used to evaluate a film-forming system containing lidocaine and prilocaine. We could also evaluate transition phases of drug compositions and quantify drugs at key times after application. Hence, the developed method may be used to provide complementary data to the Franz diffusion cell method, in terms of drug and penetration enhancer behaviors incorporated into film-forming delivery systems. (AU)

• FAPESP's process: 15/26138-4 - Development and evaluation of precursor compositions of in situ films containing anesthetic for Herpes simplex treatment.
• Grantee: Osvaldo de Freitas
• Support Opportunities: Regular Research Grants