Visible light plasmon excitation of silver nanoparticles against antibiotic-resistant Pseudomonas aeruginosa

Author(s)

da Silva, Rafael T. P. ; Petri, Marcos, V ; Valencia, Estela Y. ; Camargo, Pedro H. C. ; de Torresi, Susana I. C. ; Spira, Beny

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 31, p. 7-pg., 2020-09-01.

Abstract

The interaction of metallic nanoparticles with light excites a local surface plasmon resonance (LSPR). This phenomenon enables the transfer of hot electrons to substrates that release Reactive Oxygen Species (ROS). In this context, the present study aimed at enhancing the antibacterial effect of citrate-covered silver nanoparticles (AgNPs) by LSPR excitation with visible LED. AgNPs possess excellent antimicrobial properties against Pseudomonas aeruginosa, one of the most refractory organisms to antibiotic treatment. The Minimum Inhibitory Concentration (MIC) of the AgNPs was 10 mu g/ml under dark conditions and 5 mu g/ml under light conditions. The combination of light and AgNPs led to 100% cell death after 60 min. Flow cytometry quantification showed that bacteria treated with LSPR-stimulated AgNPs displayed 4.8 times more ROS. This significant increase in ROS possibly accounts for most of the antimicrobial effect of the AgNPs. In addition, light exposition caused a small release of silver ions (0.4%) suggesting that silver ions may play a secondary role in P. aeruginosa death. Overall, the results presented here show that LSPR stimulation of AgNPs by visible light enhances the antimicrobial activity of silver nanoparticles and can be an alternative for the treatment of topic infections caused by antibiotic-resistant bacteria such as P. aeruginosa. (AU)

• FAPESP's process: 18/16219-5 - Origin and fate of PHO-constitutive mutants in Escherichia coli
• Grantee: Beny Spira
• Support Opportunities: Regular Research Grants