Hypoglycemic active principles from the leaves of Bauhinia holophylla: Comprehensive phytochemical characterization and in vivo activity profile

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Saldanha, Luiz Leonardo ; Delgado, Aislan Quintiliano ; Marcourt, Laurence ; Camaforte, Nathalia Aparecida de Paula ; Vareda, Priscilla Maria Ponce ; Ebrahimi, Samad Nejad ; Vilegas, Wagner ; Dokkedal, Anne Ligia ; Queiroz, Emerson Ferreira ; Wolfender, Jean-Luc ; Bosqueiro, Jose Roberto

• Document type: Journal article
• Source: PLoS One; v. 16, n. 9, p. 20-pg., 2021-01-01.

Abstract

Bauhinia holophylla leaves, also known as "pata-de-vaca", are traditionally used in Brazil to treat diabetes. Although the hypoglycemic activity of this medicinal plant has already been described, the active compounds responsible for the hypoglycemic activity have not yet been identified. To rapidly obtain two fractions in large amounts compatible with further in vivo assay, the hydroalcoholic extract of B. holophylla leaves was fractionated by Vacuum Liquid Chromatography and then purified by medium pressure liquid chromatography combined with an in vivo Glucose Tolerance Test in diabetic mice. This approach resulted in the identification of eleven compounds (1-11), including an original non-cyanogenic cyanoglucoside derivative. The structures of the isolated compounds were elucidated by nuclear magnetic resonance and high-resolution mass spectrometry. One of the major compounds of the leaves, lithospermoside (3), exhibited strong hypoglycemic activity in diabetic mice at the doses of 10 and 20 mg/kg b.w. and prevents body weight loss. The proton nuclear magnetic resonance (H-1 NMR) quantification revealed that the hydroalcoholic leaves extract contained 1.7% of lithospermoside (3) and 3.1% of flavonoids. The NMR analysis also revealed the presence of a high amount of pinitol (4) (9.5%), a known compound possessing in vivo hypoglycemic activity. The hypoglycemic properties of the hydroalcoholic leaves extract and the traditional water infusion extracts of the leaves of B. holophylla seem thus to be the result of the activity of three unrelated classes of compounds. Such results support to some extent the traditional use of Bauhinia holophylla to treat diabetes. (AU)

• FAPESP's process: 16/18628-4 - Lithospermoside, a non-cyanogenic cyanoglucoside obtained from the leaves of Bauhinia holophylla (Bong.) Steud. with a potent hypoglycemiant effect: an study about the mechanism of action upon the liver.
• Grantee: José Roberto Bosqueiro
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/52237-9 - Standardized herbal medicines for the treatment of chronic diseases
• Grantee: Wagner Vilegas
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants