Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro

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Reis, Fabiana Navas ; Francese, Monique Malta ; Gomes da Silva, Natara Dias ; Pela, Vinicius Taioqui ; Frazao Camara, Joao Victor ; Trevizol, Juliana Sanches ; Pieretti, Joana Claudio ; Seabra, Amedea Barozzi ; Pessan, Juliano Pelim ; Honorio, Heitor Marques ; Crusca, Edson ; Marchetto, Reinaldo ; Rabelo Buzalaf, Marilia Afonso

• Document type: Journal article
• Source: JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS; v. 137, p. 8-pg., 2023-01-01.

Abstract

The effect of gels containing a statherin-derived peptide (Stn) on the protection against enamel and dentin erosive tooth wear (ETW) in vitro was evaluated. Bovine enamel and dentin specimens were divided into 2 groups (n = 15 and 18/group for enamel and dentin, respectively) that were treated with Chitosan or Carboxymethylcellulose (CMC) gels containing Stn15pSpS at 1.88 x 10-5 M or 3.76 x 10-5 M. Chitosan or CMC gels without active ingredients served as negative controls, while chitosan gel containing 1.23% F (as NaF) and acidulated phosphate fluoride gel (1.23% F) served as positive controls. The gels were applied on the specimens for 4 min. Stimulated saliva was collected from 3 donors and used to form a 2-h acquired pellicle on the specimens. Then, the specimens were submitted to an erosive pH cycling protocol 4 times/day for 7 days (0.01 M HCl pH 2.0/45 s, artificial saliva/2 h, and artificial saliva overnight). The gels were applied again during pH cycling, 2 times/day for 4 min after the first and last erosive challenges. Enamel and dentin loss (mu m) were assessed by contact profilometry. Scanning electron microscopy (SEM) was analyzed using a cold field emission. Data were analyzed by two-way ANOVA (for chitosan and CMC gels, separately) and Tukey's multiple comparison test. SEM images showed changes to enamel topography after application oft the gels containing Stn or F. Regarding CMC-based gels, for enamel, none of the treatments significantly reduced ETW in comparison with placebo; for dentin, however, gels containing Stn, regardless the concentration, significantly reduced the ETW. Moreover, Chitosanbased gels, regardless the Stn concentration, were able to protect enamel and dentin against ETW. Gels containing Stn might be a new approach to protect against ETW. (AU)

• FAPESP's process: 19/26070-1 - Modulation of acquired enamel pellicle to control dental mineral loss: unravelling mechanisms to make therapies possible
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 20/01634-7 - Effect of a statherin-derived peptide (Stn15pSpS) on the prevention of erosive wear of enamel and dentine in vitro
• Grantee: Fabiana Navas Reis
• Support Opportunities: Scholarships in Brazil - Master